Are you interested in achieving efficient silencing of transposable elements using CRISPRi? 🧬 🧫
Go ahead and have a look at this STAR protocols where Adami et al. share all the tips & tricks they use in the Jakobsson lab.
@anitaada.bsky.social @raquelgarza.bsky.social @jakobssonlab.bsky.social
www.cell.com
Here, we present a workflow for transcriptional silencing of transposable elements
(TEs) in human induced pluripotent stem cells (hiPSCs). We describe steps for designing
guide RNAs (gRNAs) to target ...
Happy to share the first research article of our team. We report that foreign DNA, such as ISD, activates DNA damage signaling mainly through DNA-PK.
@ircan.bsky.social @univcotedazur.bsky.social @cnrsbiologie.bsky.social @canceropolesud.bsky.social
www.jbc.org/article/S002...
If you want to explore ncRNA dynamics during mammalian development, love microscopy, and are not afaraid of transposons biology, apply‼️
We are looking for a postdoc to join our team at @imbavienna.bsky.social
More details 👇🏻
Excited to share our Nature Genetics Research Briefing on how the repetitive genome helps shape leukemia stemness.
We found that transposable elements sustain stem-like states in AML and predict patient outcomes.
www.nature.com/articles/s41...
🚨📢📄 Article in press in Genome Biology doi.org/10.1186/s130...
We introduce panREPET, a reference-free pipeline to detect shared transposable element (TE) insertions across pangenomes and retrace their evolutionary dynamics #TEsky 🧵👇
Today in
@science.org:
We are pleased to present our last work entitled:
"Concurrent L1 retrotransposition events promote reciprocal translocations in human tumorigenesis"
by Zumalave et al.
www.science.org/doi/10.1126/...
By mapping chromatin accessibility over transposable elements (TEs) across normal hematopoietic cells and the various cell types of primary acute myeloid leukemia (AML), we identified TE subfamilies w...
www.nature.com
In eukaryotic cells, DNA is normally confined in the nucleus and mitochondria and
the presence of DNA in the cytoplasm is a danger signal that activates innate immune
responses. Upon detection of cyto...
Background The role of transposable elements (TEs) in host adaptation has gained interest in recent years. Individuals of the same species undergo independent TE insertions, providing genetic variabil...
doi.org
LINE-1 (L1) retrotransposition generates somatic genomic variation in human cancer, but short-read sequencing has limited our understanding of its structural consequences and dynamics. Using long-read...
The function of nuclear speckles is revealed! This is an incredibly important paper with absolutely beautiful data! Wow! www.cell.com/cell/fulltex...
Nuclear speckles are key subnuclear structures that regulate gene expression in GC-rich
regions. This work shows that the evolution and expansion of core speckle proteins
were crucial for the increase...
We are hiring! Join the Jachowicz lab as a postdoctoral researcher. The project focuses on understanding the dynamics of ncRNAs using high-resolution and single-molecule microscopy approaches. Link below 👇
