Second preprint of the year in which @sarahgersing.bsky.social from @rhp-lab.bsky.social mapped the effects of >7500 variants in glucokinase (GCK) on the interaction with the glucokinase regulatory protein so that we now have a 3D GCK scan (abundance, interaction, activity)
doi.org/10.64898/202...
New preprint!
Led by @sarahgersing.bsky.social we map how 7,500+ variants in glucokinase (GCK) affect binding to GKRP and disentangle this from stability.
We now have activity, abundance, and interaction scores for 7,128 GCK variants - a resource for understanding phenotypes and glucose homeostasis.
With the @lindorfflarsen.bsky.social group we present our map of degrons in all human transcription factors, incl. examples of constitutive degrons in exposed regions & buried degrons that are exposed upon mutation. In addition, we show that most TADs overlap with degrons. Work led by Fia Larsen.
In our newest preprint we map degrons for >5,000 cytosolic proteins and we develop a degron predictor that can predict novel degron formation, a potential mechanism of pathogenicity. Many thanks to @rhp-lab.bsky.social, @lindorfflarsen.bsky.social and @dougfowler.bsky.social for their supervision.
In collaboration with the @lindorfflarsen.bsky.social group we release our map of degrons in >5,000 human cytosolic proteins with >99% coverage. A machine learning model trained on the data identifies missense variants forming degrons in exposed & disordered regions. Work led by @vvouts.bsky.social.
Do you think that cooperativity requires more than one binding site or subunit? Or just want to read about an interesting enzyme for which both gain- and loss-of-function variants can cause metabolic disease?
Then check out @sarahgersing.bsky.social's review on glucokinase: doi.org/10.1016/j.ti...
Many thanks to @rhp-lab.bsky.social, @lindorfflarsen.bsky.social and Torben Hansen for the collaboration, and to the editor and reviewers for their constructive feedback.
Happy to share our review, 'Glucokinase: from allosteric glucose sensing to disease variants', published in @cp-trendsbiochem.bsky.social, which explores the fascinating enzyme glucokinase. authors.elsevier.com/a/1kN5M3S6Gf...
In our new paper we characterize the effects of indel variants on protein folding and stability using a new yeast-based protein folding sensor. The work was led by Sven Larsen-Ledet and supported by the @novo-nordisk.bsky.social.
Link to paper: www.cell.com/structure/fu...