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New method paper out today on FLIM analysis with Flipper-TR. We discuss fitting strategies, common pitfalls, and quantitative interpretation for measurements. First corresponding/last authorship for me! Free link: authors.elsevier.com/a/1me2hHRzCb... Thanks to @rouxlab.bsky.social and Tithi Mandal!

3mo

I am super happy and honored to see some of my movies making it into the NYT. I hope everybody enjoys watching them go as much as I do!

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All what you want to know about flipper probes without daring asking

Exhaustive guide about Flipper TR FLIM analysis we made with Tithi Mandal and @rouxlab.bsky.social very soon in the methods in Enzymology series edited by @jeremybaskin.bsky.social

4mo

This monday we had our monthly Sciences Club. Here Cesar Bernat is showing his work on CHMP1A in mammalian cells in the @rouxlab.bsky.social ! #postdoclife

Juanma García-Arcos

3mo

Exhaustive guide about Flipper TR FLIM analysis we made with Tithi Mandal and @rouxlab.bsky.social very soon in the methods in Enzymology series edited by @jeremybaskin.bsky.social

4mo

Juanma García-Arcos

Philipp Radler

Juanma García-Arcos

thanks for showing this movie! The gradients of tension reorient while migrating cells change directions.

So grateful to @epimechfc.bsky.social for citing our work! It’s a classic from the lab, forever thanks to @colomlab.bsky.social

Congrats to Nicolas, Mathieu ad Claire for this important achievement. This will help the community tremendously!

Association of Postdoc at University of Geneva

4mo

Scientists have seen Asgard archaea crawling for the first time. When it comes to the origin of eukaryotes, this is like seeing a feathered dinosaur in the wild. (Video courtesy of Philipp Ralder)

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@mhakala.bsky.social shows that HRS forms bidimensional condensates onto endosomal membranes to flatten clathrin/ESCRT-0, an observation unexplained since the 2000s. Thx to Kukulski lab, @stefvass.bsky.social @colomlab.bsky.social @kaksonen.bsky.social @mishakudryashev.bsky.social rdcu.be/fihKk

Video

You should also read Juanma’s paper, which visualizes membrane tension gradients in migrating and non-migrating cells! rdcu.be/e2u20 It also shows that flipper truly reports tension, but that response varies with lipid composition.

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Roux Lab, Geneva

Carl Zimmer

Roux Lab, Geneva

rdcu.be

Nature Communications - This study shows that adherent cells maintain membrane tension gradients even without moving. Using a fluorescent probe, the authors reveal that actin and adhesion forces...

Adherent cells sustain membrane tension gradients independently of migration

Mandal, T., Roux, A., & García-Arcos, J. M. (2025). Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM. bioRxiv. #EpithelialMechanics buff.ly/NAytZaR

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📄 Tension gradients during migration. Using Flipper-TR FLIM, we showed that membrane tension gradients exist in all adherent cells and are actively maintained by actin and adhesion, not passively equilibrating. @rouxlab.bsky.social and Aumeier lab 🔗 bsky.app/profile/epim...

Little reminder about this paper out there, it's a great reference for anyone working on Flipper-TR experiments! By @chloeroffay.bsky.social @rouxlab.bsky.social

4mo

📄 The first study from @rouxlab.bsky.social in cells showed that planarizable Flipper probes report lipid composition, packing and phase state in GUVs, and respond to tension via changes in lipid order, both in model membranes and cells. @colomlab.bsky.social 🔗 bsky.app/profile/epim...

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Video

Juanma García-Arcos

Congrats to all authors: @dedenonmathieu.bsky.social, @rouxlab.bsky.social and especially Nicolas Rembert for pushing this project from his internship through his PhD!

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