Duke University | Tobin Lab Postdoc | PharmD, PhD
Translational Scientist interested in Cell Biology — Genetics — Microbiology — Pharmacy — Immunology — Zebrafish — Tuberculosis
Opinions are my own & don't represent those of my employer.
Charlie Pyle
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Very excited about our recent preprint- @lindashuyingxu.bsky.social and collabs discover that neutrophils repurpose their nucleolus for storage of pre-made IFN-I to be rapidly released via a nucleolar stress response that results in nucleolar extrusion and secretion.
www.biorxiv.org/content/10.6...
Discovery of gene functions in #Mycobacterium #tuberculosis has been slow. This study tests a genome-wide barcoded #transposon library across 95 environmental conditions, providing a rich resource of new gene functions, including metabolic & resistance pathways @plosbiology.org 🧪 plos.io/4vG0mz1
#OTD in 1964, Yvonne Barr co-discovered the Epstein-Barr virus, the first known 𝘩𝘶𝘮𝘢𝘯 tumor virus.
She cultured the cell lines that allowed the virus be seen. Proving viruses cause human cancer is "one of the 20th century's most significant scientific discoveries."
#WomenInSTEM #WomensHistoryMonth
Antigen-stabilizable fluorescent nanobodies that become fluorescent upon binding intracellular targets enable background-free multicolor imaging and biosensing in living systems. @sdeoliveira.bsky.social
www.nature.com/articles/s41...
New research from Duke University School of Medicine and UNC‑Chapel Hill shows how TB bacteria survive inside granulomas by activating stress‑response genes. The findings offer new insight into long‑term infection.
🔗 Read more: https://medschool.duke.edu/news/genes-help-tb-survive
#MedSky
www.biorxiv.org
A collection of antigen-stabilizable fluorescent nanobodies becomes fluorescent upon binding to their intracellular targets and enables background-free multicolor imaging and biosensing in living syst...
Clinical trial now recruiting!
clinicaltrials.gov/study/NCT071...
Cool new granuloma findings we just published in JCF in collaboration with Ravi Karra’s Lab, here at Duke. Tilmanocept, a cancer diagnostic, can be repurposed to identify cardiac sarcoidosis granulomas, which could be a noninvasive alternative to diagnostic biopsy!
onlinejcf.com/article/S107...
An interesting new study, by Clare Smith’s lab and me, here at Duke. We found that during Mtb infection, CCR6 knockout mice (on a B6 background) form necrotic pulmonary granulomas, have increased IL17 production and have sex specific differences in susceptibility.
journals.asm.org/doi/10.1128/...
What would happen if Darwin, Russell Wallace and Koch walked into a bar?
We present our new way to study mycobacteria, employing a macroevolution framework (species not strains). Possibilities are endless!! Thanks to Fernanda Subtil for pushing this.
elifesciences.org/articles/101...
Nature Methods
Admirable Women
PLOS Biology
Duke University School of Medicine
All currently approved antibiotics inhibit essential cellular processes. Ever wonder if we could kill bacteria using the opposite strategy?
Here, we demonstrate an alternative antibacterial strategy: lethality through pathway over-activation.
www.nature.com/articles/s41...
(1 out of 3)
Charlie Pyle
Using macroevolution, species not strains, uncovered important diversity in how mycobacteria respond to and resist antibiotics, revealing novel resistance determinants.
Here, the authors report metabolic activation as a potential antimicrobial strategy against Mycobacterium tuberculosis. They show that supra-physiological activation of L-histidine biosynthesis r...
An estimated 10.8 million people worldwide were infected with tuberculosis (TB) in
2023, and among those cases, 1.25 million people succumbed to disease (1); 90% of people who become infected with Myc...
journals.asm.org
Sarcoidosis is a systemic, inflammatory disease defined by the presence of noncaseating
granulomas in affected organs. Single-cell and spatial profiling have resolved granulomas
to be highly ordered s...
