🚨It's finally out!🥂 | A few years after solving the structure of human cis-PT, we now tackle its dynamics in
@NatureComms
, revealing that a conserved interfacial loop serves as an allosteric hub, communicating with the active site to regulate catalysis. rdcu.be/eShjl
Great collaboration (as always)! And very funny story how HDX and native MS complement each other and reveal details about ions, substrates and mutations. We enjoyed this ride!
Proud of this collaboration - our third paper on cisPT! Loved this one the most: a beautiful mix of high-res, low-res, and computational approaches. Grateful for this long-standing, truly fruitful team effort. Congrats to everyone!
🎉Congratulations to the SPIDoc's teams and colleagues for the recent publication
"X-ray spectroscopy meets native mass spectrometry: probing gas-phase protein complexes"
👉https://doi.org/10.1039/D5CP00604J
🔥Selected as 2025 Physical Chemistry Chemical Physics HOT article🔥 @rsc.org
Utilization of high-resolution mass spectrometry and data-independent analysis to track the monoclonal antibody spatial stability
Expert Rev Proteomics. 2025. Prochazkova V, Rosulek M, Karpisek M, Matousek V, Novak P, Kukacka Z. @peterslab1.bsky.social
doi.org/10.1080/1478...
#MassSpec #Charlesuni
This was literally cool work. ❄️ We locked ourselves in the cold room to slow down the HDX and catch even the fastest dynamics. Bonus: It turns out you can manually capture a 2-second exchange reproducibly (next time we set up the quench flow!).
Whenever we get a bioRxiv alert, we'are always curious to see what it brings. This time, the timing couldn’t have been better - a very pleasant surprise! 😄
biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Day 1 at the Structural MS Workshop in Biocev!
Straight into the lab! Hands-on HDX-MS, cross-linking, and native top-down MS.
🧪 ⚡ ❤️
Thanks to @spidocs.bsky.social, #OPAJK #Photomachines, @brukercorporation.bsky.social @ciisbinstructcz.bsky.social for the support!
#Biocev @mbuavcr.bsky.social
Free structural MS hands-on workshop @ BIOCEV (CZ)
📅 Sept 15–19, 2025
For PhDs, postdocs & undergrads (CZ/SK)
HDX-MS, native MS, cross-linking, high-res MS, data processing
Apply by July 31 → bit.ly/3HNyzIg
@spidocs.bsky.social @mbuavcr.bsky.social @ciisbinstructcz.bsky.social
Get ready to take the next step in your career journey! 🚀 Join us for the SPIDoc's Career Day, connect with professionals and discover diverse career pathways.
When: May 16, 2025.
Where: Biocev, CZ or online.
For details, see the flyer peterslab.org/downloads/SP...
Nature Communications - This study shows how the NgBR subunit controls the activity of human cis-prenyltransferase, a key enzyme for protein glycosylation. Combined experimental and computational...
🚨It's finally out!🥂 | A few years after solving the structure of human cis-PT, we now tackle its dynamics in
@NatureComms
, revealing that a conserved interfacial loop serves as an allosteric hub, communicating with the active site to regulate catalysis. rdcu.be/eShjl
SPIDoc's - MSCA Doctoral Network
Excited to share our new paper in @febsj.bsky.social! 🎉
We show how a #MYELOMA-linked GGPPS mutation tweaks hexamerization, substrate binding and product inhibition, revealing an unexpected layer of protein prenylation regulation
🔗 doi.org/10.1111/febs...
@peterslab1.bsky.social
peterslab (BIOCEV,CZ)
Mikrobiologický ústav AV ČR
peterslab (BIOCEV,CZ)
peterslab (BIOCEV,CZ)
peterslab (BIOCEV,CZ)
peterslab (BIOCEV,CZ)
Nature Communications - This study shows how the NgBR subunit controls the activity of human cis-prenyltransferase, a key enzyme for protein glycosylation. Combined experimental and computational...
Geranylgeranyl diphosphate synthase (GGPPS) plays a key role in multiple myeloma biology. Using crystallography, mass spectrometry, and fluorescence spectroscopy, this study shows that a somatic mult...
doi.org
🚨New preprint alert! Wonder how a cancer-associated mutation in GGPPS impacts hexameric stoichiometry, substrate affinity, and product inhibition? Our results shed light on a novel regulatory mechanism in protein prenylation bit.ly/3ZR7w47 #MultipleMyeloma
Protein prenylation regulates the cellular localization of small GTPases and is pivotal for multiple myeloma (MM) pathology. Geranylgeranyl diphosphate synthase (GGPPS), synthesizing a prenylation moi...
bit.ly
peterslab.org
💃🕺Happy to share a team effort to uncover the molecular "dance" behind human cis-prenyltransferase regulation!🔬🧬 @peterslab1.bsky.social @AlbGiacomello
#Biochemistry #StructuralBiology
@TAUMedFaculty @of_sagol biorxiv.org/content/10.1...
HaitinLab
Human cis-prenyltransferase (hcis-PT) synthesizes long-chain isoprenoids essential for N-linked protein glycosylation. This heteromeric complex comprises the catalytic subunit DHDDS and the regulatory...
