New preprint out 🌱
We present a new T-DNA vector system for Arabidopsis that supports clean, genomically mapped, single-copy T-DNA insertion with predictable cell-type/conditional gene expression.
@mokhalil.bsky.social + Gehring labs
biorxiv.org/content/10.6...
🧵1/16
1/2 The regulatory labyrinth for commercialisation of GE bacteria for deliberate env release eg bioremediation, biofertilization or disease control in the EU. Note that each of the 27 member states must emit an opinion & a vote 😱. No wonder the field is altogether stagnant at this side of the pond ☹️
A new tool for engineering lytic phages of the Gram-positive model bacterium Bacillus subtilis:
| ACS Synthetic Biology pubs.acs.org/doi/10.1021/...
#subtiwiki
biorxiv.org
Agrobacterium-mediated transformation is the dominant method for plant transgenesis, yet it frequently produces multi-copy, structurally complex T-DNA insertions associated with transgene silencing, u...
Phage genome engineering methods accelerate the study of phage biology, the discovery of new functions, and the development of innovative genetic engineering tools. Here, we present QuickPhage, a rapi...
A single-cell screening platform accelerates functional genetics in plants - @sattelylab.bsky.social go.nature.com/4n8wzLv
New OA Article: "In situ architecture of plasmodesmata in Physcomitrium resolved by cryo-electron tomography" rdcu.be/fjjiK
Plasmodesmata are gated by cell wall remodelling; helical protein assemblies scaffold internal membranes; implications for molecular filtering.
A pooled, cell-based, genetic screening platform in plants is used for the functional analysis of cytokinin signaling proteins.
Phages modulate plant microbiomes through bacterial lysis and by equipping bacteria
with auxiliary genes through lysogeny. In this Forum, we highlight the underappreciated
role of phages in plant heal...
www.cell.com
Cell Host & Microbe
Nature Plants
Excited to share our new preprint "Genetic code expansion enables plant-directed control of bacterial activity", a fantastic collaboration with @kunjapur.bsky.social lab!
www.biorxiv.org/content/10.6...
New Article: "Rice roots recruit Bacillus via the secretion of heptadecanoic acid" rdcu.be/ff7vo
With Research Briefing: rdcu.be/ff2X0
Rice plants facing fungal infection mobilize a soil-borne defence force via a previously unknown mechanism. MAPK inside.
www.biorxiv.org
🌾🧬 Rice blast triggers a MAPK pathway that activates OsWRKY transcription factors. These turn on OsKCS2, which makes heptadecanoic acid. This root secretion recruits beneficial Bacillus bacteria.
Field trials show higher Bacillus leads to better disease resistance. www.nature.com/articles/s41...
Jenn Brophy
This study reveals that rice roots secrete heptadecanoic acid to recruit beneficial Bacillus for defence against blast fungus, a process regulated by a MAPK pathway.
Don’t reach for ChatGPT Images or Nano Banana as your first option when creating scientific images
There are multiple free and open-source images, icons, and tools for creating scientific illustrations. #plantscience @stephenturner.us
blog.stephenturner.us/p/free-open-...
Phylopic, NIH Bioart, Bioicons, Scidraw, Open Science Art, Health Icons, Servier Medical Art, Biodiversity Heritage Library, the Noun Project, Segment Anything, Excalidraw, draw.io, Biographics
