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New preprint out šŸŒ± We present a new T-DNA vector system for Arabidopsis that supports clean, genomically mapped, single-copy T-DNA insertion with predictable cell-type/conditional gene expression. @mokhalil.bsky.social + Gehring labs biorxiv.org/content/10.6... šŸ§µ1/16
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Agrobacterium-mediated transformation is the dominant method for plant transgenesis, yet it frequently produces multi-copy, structurally complex T-DNA insertions associated with transgene silencing, u...
biorxiv.org
Rational design of T-DNA vectors enables predictable, single-copy integration in Arabidopsis thaliana
Will Shaw