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New preprint out 🌱 We present a new T-DNA vector system for Arabidopsis that supports clean, genomically mapped, single-copy T-DNA insertion with predictable cell-type/conditional gene expression. @mokhalil.bsky.social + Gehring labs biorxiv.org/content/10.6... 🧵1/16
5d
Agrobacterium-mediated transformation is the dominant method for plant transgenesis, yet it frequently produces multi-copy, structurally complex T-DNA insertions associated with transgene silencing, u...
biorxiv.org
Rational design of T-DNA vectors enables predictable, single-copy integration in Arabidopsis thaliana
Will Shaw
New OA Article: "In situ architecture of plasmodesmata in Physcomitrium resolved by cryo-electron tomography" rdcu.be/fjjiK Plasmodesmata are gated by cell wall remodelling; helical protein assemblies scaffold internal membranes; implications for molecular filtering.
29d
Nature Plants
A single-cell screening platform accelerates functional genetics in plants - @sattelylab.bsky.social go.nature.com/4n8wzLv
1mo
A pooled, cell-based, genetic screening platform in plants is used for the functional analysis of cytokinin signaling proteins.
go.nature.com
A single-cell screening platform accelerates functional genetics in plants - Nature Biotechnology
A new tool for engineering lytic phages of the Gram-positive model bacterium Bacillus subtilis: | ACS Synthetic Biology pubs.acs.org/doi/10.1021/... #subtiwiki
1/2 The regulatory labyrinth for commercialisation of GE bacteria for deliberate env release eg bioremediation, biofertilization or disease control in the EU. Note that each of the 27 member states must emit an opinion & a vote 😱. No wonder the field is altogether stagnant at this side of the pond ☹️
🌾🧬 Rice blast triggers a MAPK pathway that activates OsWRKY transcription factors. These turn on OsKCS2, which makes heptadecanoic acid. This root secretion recruits beneficial Bacillus bacteria. Field trials show higher Bacillus leads to better disease resistance. www.nature.com/articles/s41...
5mo
Phages as ecosystem engineers of plant microbiomes Forum highlighting role of phages in plant health via their eco-evolutionary effects on plant-associated bacteria, which can determine balance between mutualistic &antagonistic plant-bacteria interactions www.cell.com/cell-host-mi...
29d
1mo
Nature Biotechnology
Don’t reach for ChatGPT Images or Nano Banana as your first option when creating scientific images There are multiple free and open-source images, icons, and tools for creating scientific illustrations. #plantscience @stephenturner.us blog.stephenturner.us/p/free-open-...
13d
1mo
blog.stephenturner.us
Phylopic, NIH Bioart, Bioicons, Scidraw, Open Science Art, Health Icons, Servier Medical Art, Biodiversity Heritage Library, the Noun Project, Segment Anything, Excalidraw, draw.io, Biographics
Free and open-source images, icons, and tools for creating scientific illustrations
New Article: "Rice roots recruit Bacillus via the secretion of heptadecanoic acid" rdcu.be/ff7vo With Research Briefing: rdcu.be/ff2X0 Rice plants facing fungal infection mobilize a soil-borne defence force via a previously unknown mechanism. MAPK inside.
Molecular Microbiology
Cell Host & Microbe
1mo
Nature Plants
Excited to share our new preprint "Genetic code expansion enables plant-directed control of bacterial activity", a fantastic collaboration with @kunjapur.bsky.social lab! www.biorxiv.org/content/10.6...
12d
www.biorxiv.org
Global Plant Science Spotlight
Global Plant Science Spotlight
Victor de Lorenzo (CNB, Madrid)
Jenn Brophy
This study reveals that rice roots secrete heptadecanoic acid to recruit beneficial Bacillus for defence against blast fungus, a process regulated by a MAPK pathway.
www.nature.com
Rice roots recruit Bacillus via the secretion of heptadecanoic acid - Nature Plants
www.cell.com
Phages modulate plant microbiomes through bacterial lysis and by equipping bacteria with auxiliary genes through lysogeny. In this Forum, we highlight the underappreciated role of phages in plant heal...
Phages as ecosystem engineers of plant microbiomes
Phage genome engineering methods accelerate the study of phage biology, the discovery of new functions, and the development of innovative genetic engineering tools. Here, we present QuickPhage, a rapi...
pubs.acs.org
Advancing Fast-Track Genome Engineering in Bacillus subtilis Phages