1) Removing external saline keeps neuronal physiology consistent over days or even weeks.
2) Our simplified holder allows flies to exhibit natural behaviors under the microscope.
3) The glue effectively stabilizes brain motion, so no need to remove muscle 16, even during feeding!
Drosophila folks! We just shared a new protocol for surgical preparation that enables long-term imaging in adult flies. It’s low-cost, simple, and highly repeatable. For demonstration, we show a 10-day voltage imaging and a 1-week population calcium imaging results.
www.biorxiv.org/content/10.6...
doi.org/10.64898/202...
Happy New Year! 🥳 CSB Friday seminars resume Jan 9 with a fascinating talk by Prof Cheng Huang @huangchengwashu.bsky.social on “Decoding dopamine-mediated learning algorithms in the Drosophila brain”! 🧪🪰
Join us at 11am in RW432!
csb.utoronto.ca/events/cheng...
Long-range axon branching: contributions to brain network plasticity and repair — a Review by Linda J. Richards, Cheng Huang, Adam Q. Bauer & Jin-Moo Lee
#neuroscience #neuroskyence
www.nature.com/articles/s41...
Recent review about long-range axons rdcu.be/eXeDG
This is our best in vivo AP propagation movie so far. Recorded at 1k Hz from the axonal branches of a fly CNS neuron, using voltage indicator pAce.