📣 Preprint alert! We developed a method to analyze concurrent interactions between multiple chromatin regions at single alleles at sub-nucleosome resolution (multi-way Micro-Capture-C, mwMCC) & used this to study structural synergy within super-enhancers. 1/14
www.biorxiv.org/content/10.6...
JIT 4 summer, the latest preprint from our lab, brilliantly led by @kaitezhang.bsky.social w/ support from Collepardo lab (@juliamaristany.bsky.social / @janhuemar.bsky.social). www.biorxiv.org/content/10.6... describes a new single-molecule epigenomic method, & discoveries it enables ... (1/n)
Nucleosome spacing influences higher-order chromatin fiber organization in vitro but how this relates to cellular chromosome structure remains contentious. To address this, we developed Ligation Analy...
Many of you may have received pessimism from me over the years about using Hi-C to predict distal regulatory effects, mired with paradoxical optimism that cohesin loop extrusion is key.
Well, the theoretical story underlying my seemingly paradoxical rambling is finally ready to be told. 👇
Please join us for the next edition of the GOA monthly seminar series Dr Karissa Hansen
Registration link: unimelb.zoom.us/webinar/regi...
🆕 ADVANCE ONLINE 🆕
RESEARCH PAPER: Cohesin-mediated loop extrusion and enhancer-associated factors additively contribute to Sox2 looping with its distal enhancer
By Martinovic et al., and Elzo de Wit
➡️ https://ow.ly/H1mH50YWiTO
Elzo de Wit lab @ NKI Netherlands Cancer Institute
#enhancer #cohesin
Interested in hearing about mechanisms of cohesin-dependent vs. -independent enhancer regulation?
@karissalhansen.bsky.social will be presenting her work
**Tues June 23rd 8am CET**
www.biorxiv.org/content/10.6...
www.science.org/doi/10.1126/...
Thank you @genome-org-aus.bsky.social !
👇
🚨🧪🧵URGENT INPUT NEEDED: NIH asks input for next strategic plan-including emphasis on non-animal models (NAMs) to replace animals. Per NIH insider, anti-animal responses now outnumber scientists 200-700x. Please please provide input. Deadline: May 26, 11:59 PM ET.
grants.nih.gov/news-events/...
Fantastic Q, we have been wondering about this too.
Bridging frequency & duration seems to be what matters most for activation. So as long as those increase somehow, chromatin around can be decompacted
@lucagiorgetti.bsky.social modeled bridging together with 3D folding and might have insight?
27/ re-upping the recent thread on highly relevant work from @lucagiorgetti.bsky.social using very different experimental + modeling approaches.
Truly foundational findings for the field!
bsky.app/profile/luca...
By @mattiaubertini.bsky.social
@nesslfy.bsky.social
