High-throughput screening approach identifies substrate-selective Hsp104 variants that counter amyloid seeding with diminished off-target effects
An unapologetically angry post about what’s being done to US science:
Ryan et al. have developed a high-throughput approach for identifying variants of the amyloid disaggregase Hsp104. Their approach facilitates analysis of large Hsp104 variant libraries in parallel and with quantitative output. Using this approach, they identified disaggregase variants that restore function to proteins that misfold in ALS and Parkinson’s disease.
"a long non-coding RNA transcribed from a DNA region in the fungal pathogen Magnaporthe oryzae translocates into host rice cells and sequesters a complementary microRNA (miRNA), derived from a distinct host DNA region, thereby subverting host immunity"
www.nature.com/articles/s41...
🌪️🩸 #Dust storms in the U.S. Southwest may transport #microbes, #fungi, and mineral particles long distances, contributing to rare “blood rain” events and #health concerns.
#PublicHealth #ClimateChange #Microbiology via @sciencefocus.bsky.social
Derek Lowe
A fungal long non-coding RNA from Magnaporthe oryzae translocates into rice cells to sequester a host microRNA that normally represses PKR1, a negative immunity regulator, thereby facilitating in...
The results here re 3D classification are quite impressive. Would be interesting to see how much the LPP improves classification of near-native samples with extreme compositional heterogeneity (e.g. derived from cellular fractions)
Cellular water-potential sensing through biomolecular condensation
www.nature.com/articles/s41...
Microbes.Info
Finally out in @Cellcellpress!
Proteins with long IDRs are prone to misfolding during protein synthesis.
This is prevented by mRNA 3′UTRs that act as mRNA-based IDR chaperones.
www.cell.com/cell/fulltex...
MoCox6 is an inner mitochondrial membrane regulator of mitophagy in Magnaporthe oryzae, and targeting it with a small molecule, Pan-RAS-IN-1, suppresses fungal virulence and reduces rice blast disease...
Highly conserved mRNA 3′ UTRs act as co-translational chaperones for intrinsically
disordered regions (IDRs), preventing inter-domain misfolding and enabling biogenesis
of fully active proteins.
Plants sense water deficiency through SAM8 protein condensation, which responds to reduced hydration and triggers stress adaptation by altering RNA export and gene translation.
Phase plates can in principle overcome the poor image contrast in electron cryo–microscopy (cryo-EM) and the resulting limits on the structural reconstruction of small proteins. However, previous desi...
In a series of 3 papers and preprints, we’re thrilled to share with you the working laser phase plate. In collaboration with research led by Holger Müller at UC Berkeley, this is a huge innovation in imaging to make small and faint objects inside cells visible. bit.ly/4vK9LVn
