High-throughput screening approach identifies substrate-selective Hsp104 variants that counter amyloid seeding with diminished off-target effects
Ryan et al. have developed a high-throughput approach for identifying variants of the amyloid disaggregase Hsp104. Their approach facilitates analysis of large Hsp104 variant libraries in parallel and with quantitative output. Using this approach, they identified disaggregase variants that restore function to proteins that misfold in ALS and Parkinson’s disease.
