Asst. Prof. Uni Groningen 🇳🇱
Comp & Exp Biochemist, Protein Engineer, 'Would-be designer' (F. Arnold) | SynBio | HT Screens & Selections | Nucleic Acid Enzymes | Biocatalysis | Rstats & Datavis
https://www.fuerstlab.com
https://orcid.org/0000-0001-7720-9
Max Fürst
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Took a while, but happy to share the published version of our article on ADDing, our highly efficient new enzymatic protein labelling platform that made it on the cover of ACS bioconjugate chemistry 😁
pubs.acs.org/toc/bcches/c...
The molecular structure of flavin transferase ApbE in complex with an ADD tag-bearing protein substrate, GFP: The transferase is depicted in the process of installing the click handle onto the target threonine, facilitated by the use of the ADD substrate that is generated enzymatically with ADP-ribosyl cyclase from NAD+. View the article.
🚨 preprint 2️⃣ this month: our (purely experimental🧪) venture into #ChemBio
We prouldy present: ADD-tagging of proteins (or "ADDing") —a super convenient enzymatic technique to install click chemistry handles on proteins.
Led by superstar @wahyuwidodo.bsky.social
www.biorxiv.org/content/10.1...
A 🧵👇🏽
well if you liked this you might also be interested in the graph I made for the same course on the abundance of elements. Green are the CHNOPS element, which constitute 98% of living matter on earth. Fun to speculate about the ones way off the axis
wtaf biology, as if you hadn't had enough enzyme mechanisms for adding oxygen already
Grep this: Microsoft grafts (most) Linux commands onto Windows
I wrote about AI in academia. "PhD-level thinking", LLM bias, grunt work, alignment, AGI, data center water use, AI politics -- something for everyone.
Max Fürst
Earlier this week I posted an example of a fake western blot provided by ThermoFisher to demonstrate the validity of a p53 antibody. I considered it an amusing curiosity. In fact Thermo Fisher Scientific has systematically manipulated antibody validation data!
Coreutils serves over 75 Unix commands in Windows and PowerShell command lines
Paying peer reviewers works.
Expanded Fast & Fair experiment @biologyopen.bsky.social:
• 5.5 vs 37.7 working days to decision with reviews
• ~3 vs ~9 reviewer invitations per manuscript
• no reduction in editor-assessed review quality
• similar acceptance rates
www.biorxiv.org/content/10.6...
Max Fürst
Max Fürst
The Register
Unconventional monooxygenation by the O2-dependent tRNA wobble uridine hydroxylase TrhO www.nature.com/articles/s41... 🦠 cryo-electron microscopy structure of Bacillus subtilis TrhO–tRNAAla complex
Screen 1M random protein sequences to discover that biology-like folds are accessible from random sequences with surprising frequency
www.biorxiv.org/content/10.6...
PhD-level thinking, LLM bias, alignment, AGI, data centers, and AI politics
Unconventional monooxygenation by the O2-dependent tRNA wobble uridine hydroxylase TrhO www.nature.com/articles/s41... 🦠 cryo-electron microscopy structure of Bacillus subtilis TrhO–tRNAAla complex
Made this graph for my undergrad lecture
Shin et al. report the cryo-electron microscopy structure and catalytic mechanism of TrhO, a cofactor-independent enzyme that hydroxylates tRNA wobble uridine using molecular oxygen through a…
Shin et al. report the cryo-electron microscopy structure and catalytic mechanism of TrhO, a cofactor-independent enzyme that hydroxylates tRNA wobble uridine using molecular oxygen through a…
