wtaf biology, as if you hadn't had enough enzyme mechanisms for adding oxygen already
well if you liked this you might also be interested in the graph I made for the same course on the abundance of elements. Green are the CHNOPS element, which constitute 98% of living matter on earth. Fun to speculate about the ones way off the axis
Took a while, but happy to share the published version of our article on ADDing, our highly efficient new enzymatic protein labelling platform that made it on the cover of ACS bioconjugate chemistry 😁
pubs.acs.org/toc/bcches/c...
The molecular structure of flavin transferase ApbE in complex with an ADD tag-bearing protein substrate, GFP: The transferase is depicted in the process of installing the click handle onto the target threonine, facilitated by the use of the ADD substrate that is generated enzymatically with ADP-ribosyl cyclase from NAD+. View the article.
pubs.acs.org
Unconventional monooxygenation by the O2-dependent tRNA wobble uridine hydroxylase TrhO www.nature.com/articles/s41... 🦠 cryo-electron microscopy structure of Bacillus subtilis TrhO–tRNAAla complex
Max Fürst
Screen 1M random protein sequences to discover that biology-like folds are accessible from random sequences with surprising frequency
www.biorxiv.org/content/10.6...
Paying peer reviewers works.
Expanded Fast & Fair experiment @biologyopen.bsky.social:
• 5.5 vs 37.7 working days to decision with reviews
• ~3 vs ~9 reviewer invitations per manuscript
• no reduction in editor-assessed review quality
• similar acceptance rates
www.biorxiv.org/content/10.6...
Shin et al. report the cryo-electron microscopy structure and catalytic mechanism of TrhO, a cofactor-independent enzyme that hydroxylates tRNA wobble uridine using molecular oxygen through a…
www.nature.com
Shin et al. report the cryo-electron microscopy structure and catalytic mechanism of TrhO, a cofactor-independent enzyme that hydroxylates tRNA wobble uridine using molecular oxygen through a…
Grep this: Microsoft grafts (most) Linux commands onto Windows
I wrote about AI in academia. "PhD-level thinking", LLM bias, grunt work, alignment, AGI, data center water use, AI politics -- something for everyone.
Zeynep Baharoglu
Zeynep Baharoglu
Max Fürst
www.theregister.com
PhD-level thinking, LLM bias, alignment, AGI, data centers, and AI politics
open.substack.com
Coreutils serves over 75 Unix commands in Windows and PowerShell command lines
🚨 preprint 2️⃣ this month: our (purely experimental🧪) venture into #ChemBio
We prouldy present: ADD-tagging of proteins (or "ADDing") —a super convenient enzymatic technique to install click chemistry handles on proteins.
Led by superstar @wahyuwidodo.bsky.social
www.biorxiv.org/content/10.1...
A 🧵👇🏽
