Took a while, but happy to share the published version of our article on ADDing, our highly efficient new enzymatic protein labelling platform that made it on the cover of ACS bioconjugate chemistry 😁
pubs.acs.org/toc/bcches/c...
The molecular structure of flavin transferase ApbE in complex with an ADD tag-bearing protein substrate, GFP: The transferase is depicted in the process of installing the click handle onto the target threonine, facilitated by the use of the ADD substrate that is generated enzymatically with ADP-ribosyl cyclase from NAD+. View the article.
🚨 preprint 2️⃣ this month: our (purely experimental🧪) venture into #ChemBio
We prouldy present: ADD-tagging of proteins (or "ADDing") —a super convenient enzymatic technique to install click chemistry handles on proteins.
Led by superstar @wahyuwidodo.bsky.social
www.biorxiv.org/content/10.1...
A 🧵👇🏽
