Here is the paper in @biochemsoc.bsky.social Bioscience Reports:
portlandpress.com/bioscirep/ar...
Congratulations to the @markohyvonen.bsky.social Group on its celebration of 25 years this week by 40 alumni! Since 2001, it has welcomed 21 Postdocs, 8 RAs, 15 PhDs, 6 Visiting PhDs, 9 MScs and 46 Undergrads and contributed over 90 publications. 🎂🎉👏 Read more: www.bioc.cam.ac.uk/news/hyvonen...
Abstract. Affinity chromatography is a powerful and therefore popular method for the purification of proteins for structural studies. The success of the technique relies on the specificity of the inte...
Ok, time to get back on this platform with some science, as exciting things are coming out. Well, I think they are exciting.
Let's start with cool FBDD story.Together with Spring group we have come up with a covalent inhibitors for PH domain. Activated lysine in PIP3 binding site, it seems.
Here is the paper itself if you want to take a peak.
doi.org/10.1021/acs....
Just out is a little cautionary tale by @emmabelcher96.bsky.social
Turns out StrepTactin pulls down biotinylated proteins from mammalian cells and anti-FLAG antibody likes its own specific contaminants. Both of which can end up dominating your precious cryo-EM images.
You've been warned!