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Thanks a lot to @atinygreencell.bsky.social for these awesome chromoprotein strains!

New preprint out 🌱 We present a new T-DNA vector system for Arabidopsis that supports clean, genomically mapped, single-copy T-DNA insertion with predictable cell-type/conditional gene expression. @mokhalil.bsky.social + Gehring labs biorxiv.org/content/10.6... 🧵1/16

2mo

🧬 Do you clone using PCR-based homologous recombination? This web tool automates primer design for deletions, C-terminal tagging, and promoter change, and can simulate the expected allele and diagnostic PCRs. 🔗 Link in the replies www.youtube.com/watch?v=TOHS...

Agrobacterium-mediated transformation is the dominant method for plant transgenesis, yet it frequently produces multi-copy, structurally complex T-DNA insertions associated with transgene silencing, u...

biorxiv.org

Rational design of T-DNA vectors enables predictable, single-copy integration in Arabidopsis thaliana

Will Shaw

“wait… it parses the history too??” yep 😄 OpenCloning now imports SnapGene files with the full cloning workflow, not just the final construct. 🎬 sound on opencloning.org

🧬 Phage recombinases just landed in hashtag#OpenCloning Simulate site-specific recombination defining your own recognition sites. 🎥 See it in action below 👇 Links in the comments 🔗

Noah Sprent

2mo

Try it here: api.opencloning.org/batch_clonin... Built with OpenCloning: opencloning.org

Video

Join us May 14, 6pm at Imperial! 🧫 Design, cloning, & competitive growth screening of CRISPRi and protein variant libraries to study yeast killer toxins Thijs de Vroet (Billerbeck Lab) 🌱 Evolving biological nitrogen fixation with microbial co-cultures Amir Guppy (Isalan Lab) shorturl.at/aGL5M

Manuel Lera-Ramirez