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šŸ§¬ Do you clone using PCR-based homologous recombination? This web tool automates primer design for deletions, C-terminal tagging, and promoter change, and can simulate the expected allele and diagnostic PCRs. šŸ”— Link in the replies www.youtube.com/watch?v=TOHS...
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YouTube video by Genestorian
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OpenCloning: Batch design of primers for PCR-based cloning in yeast
Manuel Lera-Ramirez