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Profile

Working on OpenCloning, an Open Source web application to plan and document cloning. https://opencloning.org/ Open Source in science enthusiast. Previously PomBase, yeast genetics, image analysis and cytoskeleton.

Manuel Lera-Ramirez

@nicolabiologist.bsky.social

3mo

Thanks a lot to @atinygreencell.bsky.social for these awesome chromoprotein strains!

2mo

Manuel Lera-Ramirez

“wait… it parses the history too??” yep 😄 OpenCloning now imports SnapGene files with the full cloning workflow, not just the final construct. 🎬 sound on opencloning.org

How the assembler works: bsky.app/profile/manu... Try it here: app.opencloning.org Docs: docs.opencloning.org/assembler/

1mo

Enabled by the library sgffp: github.com/merv1n34k/sg...

3mo

Try it here: app.opencloning.org Docs: docs.opencloning.org/methods/phag... Project website: opencloning.org

🧬 Just pushed new updates to #OpenCloning Assembler based on user feedback! ✍️ Now supports #GoldenBraid and Plant #MoClo 🚀 You can edit assembly product names, view a full syntax overview, and enjoy cleaner visuals. 👉 Try it out (link in comments) — feedback welcome!

1mo

2mo

3mo

Manuel Lera-Ramirez

🧬 Phage recombinases just landed in hashtag#OpenCloning Simulate site-specific recombination defining your own recognition sites. 🎥 See it in action below 👇 Links in the comments 🔗

Manuel Lera-Ramirez

2mo

Noah Sprent

Manuel Lera-Ramirez

Join us May 14, 6pm at Imperial! 🧫 Design, cloning, & competitive growth screening of CRISPRi and protein variant libraries to study yeast killer toxins Thijs de Vroet (Billerbeck Lab) 🌱 Evolving biological nitrogen fixation with microbial co-cultures Amir Guppy (Isalan Lab) shorturl.at/aGL5M

1mo