Sort of shocked that Sm proteins aren't mentioned in the paper. The U1 snRNA site they identify overlaps with the Sm protein binding site...Seems important? In addition Figure 4j is a bit surprising...
We just published our new paper about an active Helitron transposon in wheat 🌾🧬
We found that it can be mobilised by heat stress when DNA methylation is reduced.
We document its full lifecycle: RNA -> circular DNA -> integration
www.nature.com/articles/s41...
#TEsky
Super excited to begin the Harden Machines on Genes meeting in beautiful Crete!
#BiochemEvent @biochemsoc.bsky.social
www.eventsforce.net/biochemsoc/f...
My lab is currently recruiting 2(!!) postdocs in RNA structural biology/biochemistry! Full details can be found below and on our website (sites.lsa.umich.edu/keane-lab/op...).
It may be real, but I'm suspecting that it may not be the same U1 involved in splicing but rather one of the other U1 variant genes (there are many in humans) that for some reason or another isn't able to assemble a Sm ring. It may be a good idea to isolate the U1/U3 hybrid & sequence the snRNA...
Alternatively it could be the pre-snRNA... again sequencing would help (or a northern blot, sigh...)
Huh... some head scratching here...
www.biorxiv.org/content/10.6...
A U1-U3 snRNA-snoRNA interaction couples SF3B1 mutation to chromatin-state rewiring and genome instability
Peng Xia, , Omar Abdel-Wahab, Bei Liu, Chuan He et al...
New paper! How do RNAs "know" where to go inside a cell? We dug into the sequence elements that route RNAs to the right place. It turns out that, in mammals, they're surprisingly massive (>200 nt), multipartite, and wonderfully complicated. 🧵