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Well done. But is GTP-tubulin in an expanded state at the tip of growing microtubules? Indeed, nobody knows 🤔
Check the little video they made from the interview (with the “Rolex” Rave skateboards tee-shirt 😆)
Nice story 😎
1mo
10d
2h
Video
Christophe 🔬 Leterrier
Denis Chrétien
Denis Chrétien
New preprint! In collaboration with the Steinmetz Lab at PSI we report cryo-EM structures of microtubules at up to 1.9 Å (!) resolution & in different nucleotide state mimics, suggesting a mechanism for lattice-induced GTP hydrolysis and why it leads to catastrophe: tinyurl.com/7cjrf863
Glad to see the work of my PhD on the manchette microtubule now out in @embojournal.org: doi.org/10.1038/s443...
13h
10d
I was interviewed by @snehakhedkar.bsky.social for The Scientist about our work using super-resolution microscopy to map the neuronal architecture, and make nice pictures along the way! Check the article here: www.the-scientist.com/super-resolu...
1mo
The new release of #BigVolumeBrowser (0.1.0) finally adds animation render functionality to it, plus some other navigation improvements and bug fixes. Here is a movie I've made for the microtubule network (via ExM) in different cell types. Contact me if you want it for educational/research purposes.
The manchette is a transient microtubule (MT)-based structure that is vital for the correct shaping of sperm during spermiogenesis. Throughout spermiogenesis, the manchette retains structural integrity for several days, raising the question of how its MTs are regulated. Here, using cryo-electron tomography of manchettes isolated from rat testes, we find that manchette MT ends are structurally diverse. We show that the MT-binding protein CLASP2 is present throughout the manchette and likely regulates both MT ends. Using cryo-electron microscopy single particle analysis and super-resolution microscopy, we reveal that SPACA9 and MNMIP1 (SH3D21) bind to the seam of manchette MTs from the luminal side. SPACA9 binds to both α- and β-tubulin of protofilament 1 but does not interact directly with protofilament 13, while MNMIP1 binds directly to protofilament 13. MNMIP1 further extends and threads through the MT lattice at the seam. Our study reveals a novel seam MT inner protein complex with a unique binding mode, providing a plausible explanation for MT regulation that maintains manchette integrity over an extended period.
doi.org
SPACA9 and MNMIP1 bridge the seam of spermatid manchette microtubules - The EMBO Journal
2mo
Video
Wieczorek Lab
Jo Judernatz
Christophe Leterrier uses advanced microscopy to uncover neurons’ intricate nanostructure and dynamics while creating striking images that bring brain science to life.
www.the-scientist.com
Super-Resolution Microscopy Helps Map Hidden Neuronal Architecture | The Scientist
La #plateforme TEM2C de l'UAR Biosit propose ses services en cryo-microscopie électronique, cryo-tomographie et cryo-CLEM pour la biologie structurale et cellulaire ➡️ www.ibisa.net/plateformes-... @cnrsbiologie.bsky.social @inserm.fr @rennesuniv.bsky.social
I love us
Christophe 🔬 Leterrier
So lovely 😍
Looks cool, will try!
8d
23d
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27d
Try this new online training game from Leandro F. Estrozi designed for newcomers in structural biology, with a focus on cryo-EM and cryo-ET map interpretation: rico.ibs.fr/helixplo...
Approches intégrées de cryo-microscopie électronique, cryo-tomographie et cryo-CLEM pour la biologie structurale et cellulaire.
www.ibisa.net
I am thrilled to share my PhD work with @nicolettapetridou.bsky.social @embl.org: www.biorxiv.org/cgi/content/... A fun collaboration with Bernat Corominas-Murtra, Adrián Aguirre-Tamaral, @bart-smeets.bsky.social , Jef Vanghel and Tom Belpaire! Read the story in the comments below ⤵️
Transmission electron microscopy, cryo and CLEM (TEM2C)
1mo
12h
Video
Denis Chrétien
Denis Chrétien
Eugene Katrukha 🇺🇦
GIS IBiSA
Leah
Lena Schindler
RCSB Protein Data Bank
This deep sea creature is as sharp & clear as broken class, & can grow as long as your hand. This giant amphipod, (Cystisoma) only has two colored body parts: the dense orange stomach/egg pouch, & two MASSIVE eyes, which are a glittering holographic orange layer completely covering its head. 📽️ MBARI
We're happy to announce our new preprint! 🐸 easymode: general pretrained networks for cellular cryo-ET. Segment ~20 cellular features – ribosomes, microtubules, mitochondria, nuclei & more – with zero model training. 🔗 doi.org/10.64898/202... 🧵👇
2mo
27d
Video
Microtubule comets in human iPSCs to kick off my first week of grad school! ☄️💫
19d
Video
Rebecca R Helm
Allegretti Lab
Sareen Fiaz