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🧵 New protocol out in @cp-starprotocols.bsky.social! Step-by-step guide to infer forces in tissues using ForSys, by @borgesaugust.bsky.social, Jeronimo Miranda, Alberto Ceccarelli, Guilherme Ventura, @kuba-sedzinski.bsky.social, @hernanlopez-schier.bsky.social and I, www.cell.com/star-protoco...
Check out this amazing conference of Mathematical Biology! Great speakers, venue and city!!
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Osvaldo Chara
Osvaldo Chara
Scripting is also easy! Load your images into Frame objects, then pass them to ForSys: import forsys as fs → build frames{} per timepoint → forsys = fs.ForSys(frames) and then infer. Works for both experimental šŸ”¬ and in silico šŸ’» data!
Would you like to run our Cellular Potts Model (#CPM) of neuromast regeneration in #zebrafish? It is now available in the amazing @morpheus-lab.bsky.social repository: morpheus.gitlab.io/model/m4377/ Curious about the Science? Check our paper: doi.org/10.1016/j.jt...
ForSys is an open-source Python tool for non-invasive stress inference from microscopy images. It comes with 3 usage options: a Fiji GUI šŸ–„ļø, a Command Line Interface āŒØļø, and full Python scripting šŸ““. Use what you need!
GUI users: install the ForSys plugin in Fiji via Help→Update→Manage Update Sites. Add sites.imagej.net/ForSys, restart, then open Plugins→ForSys. Point it to your image folder, set your conda environment, and hit Run! No coding needed.
Thanks to all co-authors, labs, and collaborators! ForSys is free & open source. Find the detailed usage description in the Protocol (www.cell.com/star-protoco... and the code, example notebooks & full docs here: github.com/borgesaugusto/forsys Happy inferring! šŸ«§šŸ”¬
CLI users: activate your forsys environment, then run one command! Static: python -m forsys -f /data -m nnls -sf /resultsv or for Dynamic inference: add –dynamic. Full flag list via -h or in the docs at forsys.readthedocs.io.
Check all details behind ForSys and its validation in our bsky.app/profile/cp-i... publication: www.cell.com/iscience/ful.... This protocol focuses on how to actually run it, step by step
Amazing paper in @pnas.org of @yanlanmao.bsky.social lab featuring @giuliapaci.bsky.social and colleagues!!
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