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Check all details behind ForSys and its validation in our bsky.app/profile/cp-i... publication: www.cell.com/iscience/ful.... This protocol focuses on how to actually run it, step by step
17d
Optical imaging
www.cell.com
ForSys: Non-invasive stress inference from time-lapse microscopy
GUI users: install the ForSys plugin in Fiji via Help→Update→Manage Update Sites. Add sites.imagej.net/ForSys, restart, then open Plugins→ForSys. Point it to your image folder, set your conda environment, and hit Run! No coding needed.
Osvaldo Chara
17d
Osvaldo Chara
sites.imagej.net
Index of /ForSys
Amazing paper in @pnas.org of @yanlanmao.bsky.social lab featuring @giuliapaci.bsky.social and colleagues!!
Scripting is also easy! Load your images into Frame objects, then pass them to ForSys: import forsys as fs β†’ build frames{} per timepoint β†’ forsys = fs.ForSys(frames) and then infer. Works for both experimental πŸ”¬ and in silico πŸ’» data!