BTW, this tutorial includes a bit of Mosaic, a membrane modeling software from the Kosinski lab a lot of tomo labs may have missed, but which I absolutely love!
New work from my team: pubs.acs.org/doi/10.1021/...
We used probes to ID targets of carbapenems in M. abscessus. 17 targets active in both carbon starved and replicating cultures. Includes validation of 9 targets. Part of a special issue ("NTM Pathogens – Biology, Infection, and Drug Discovery).
Very excited about our new work highlighting the role of lipids in mediating multi-channel assemblies of Cx-46/50!
BIGSMALL is now implemented in SPACEtomo.
Setup is easy:
- define low mag parameters with SerialEM Search preset
- add LM + HM targets in the same beam-shift group
- acquire!
(5/6)
Interested in microbiology, immunology, and host-pathogen interactions? Come work with us!
Our department at OHSU is opening a search for a new tenure-track faculty position. See the job advertisement for details.
Please share within your networks!
www.nature.com/naturecareer...
The deadline to register for the 2026 Cryo-ET Workshop has been extended extended to April 10! Learn more about this hands-on workshop and apply at: myumi.ch/lsi-cryo-workshop-2026
@jbquerido.bsky.social @shyamalm.bsky.social
Kvelling (reverse kvelling?) for my mom today!
My day of the class used the 2.0.0beta1 release of surface morphometrics, which is packed full of a TON of new features, and is 40-50x faster than the 1.0 release. Expect a manuscript later this summer!
I had such a fun time teaching at the U Michigan Cryo-ET Course this week! I taught quantitative ultrastructure analysis and we made cool figures like these! My course materials are at baradlab.com/michigan_tutorial for anyone to try. Workflow include mesh refinement and thickness measurement!
