Circular RNA-mediated inverse prime editing in human cells
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Prime editor with rational design and AI-driven optimization for reverse editing window and enhanced fidelity
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Feedback-responsive cell factories for dynamic modulation of the unfolded protein response
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Generative and predictive neural networks for the design of functional RNA molecules
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Sucrose-driven carbon redox rebalancing eliminates the Crabtree effect and boosts energy metabolism in yeast
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Precision multiplexed base editing in human cells using Cas12a-derived base editors
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Spatially blocked split CRISPR-Cas12a system for ultra-sensitive and versatile small molecule activation and detection
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Designer artificial environments for membrane protein synthesis
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A generalizable approach for programming protease-responsive conformationally inhibited artificial transcriptional factors
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A chimeric viral platform for directed evolution in mammalian cells
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Designing high performance RNA molecules is a unifying challenge across many areas of biotechnology research. Here, authors develop GARDN and SANDSTORM, a data-efficient generative AI framework for de...
Expanding editing scope while reducing byproducts is now possible for prime editing. Here, authors develop reverse prime editing (rPE), a SpCas9-based tool enabling editing in reverse window with high...
Prime editors are restricted to performing precise edits downstream of cleavage sites. Here, authors develop helicase-assisted circular RNA-mediated inverse PEs (ciPEs) to increase editing efficiencie...
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Engineering cell factories that support the production of large quantities of protein therapeutics remains a significant biomanufacturing challenge. Here the authors engineered cells to sense an early...
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The Crabtree effect affects yeast-based bioproduction. Here, the authors report the development of a Crabtree-negative yeast through introducing the sucrose phosphorolysis pathway, deletion of the pho...
Current base editors cannot simultaneously edit multiple loci with base-pair level precision, hindering complex genotype generation. Here the authors describe optimized Cas12a gRNA array designs, enab...
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Detecting small molecules is pivotal across many fields. Here, authors leverage a split crRNA mode to construct spatially blocked split CRISPR-Cas12a system. This approach establishes a low-background...
Membrane proteins are notoriously difficult to synthesize. Here, authors introduce MEMPLEX, a high-throughput experimentation platform guided by machine learning that designs artificial cell-free envi...
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Artificial transcription factors (ATFs) are core elements of customized circuits responsible for signal output and event transformation. Here, the authors report the design of a protease-responsive co...
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Directed evolution is a process of mutation and artificial selection to breed biomolecules with new or improved activity. Here the authors develop a directed evolution platform (PROTein Evolution Usin...
