Precision multiplexed base editing in human cells using Cas12a-derived base editors
www.nature.com/articles/s41...
Sucrose-driven carbon redox rebalancing eliminates the Crabtree effect and boosts energy metabolism in yeast
www.nature.com/articles/s41...
Spatially blocked split CRISPR-Cas12a system for ultra-sensitive and versatile small molecule activation and detection
www.nature.com/articles/s41...
Circular RNA-mediated inverse prime editing in human cells
www.nature.com/articles/s41...
Prime editor with rational design and AI-driven optimization for reverse editing window and enhanced fidelity
www.nature.com/articles/s41...
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Expanding editing scope while reducing byproducts is now possible for prime editing. Here, authors develop reverse prime editing (rPE), a SpCas9-based tool enabling editing in reverse window with high...
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The Crabtree effect affects yeast-based bioproduction. Here, the authors report the development of a Crabtree-negative yeast through introducing the sucrose phosphorolysis pathway, deletion of the pho...
Prime editors are restricted to performing precise edits downstream of cleavage sites. Here, authors develop helicase-assisted circular RNA-mediated inverse PEs (ciPEs) to increase editing efficiencie...
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Detecting small molecules is pivotal across many fields. Here, authors leverage a split crRNA mode to construct spatially blocked split CRISPR-Cas12a system. This approach establishes a low-background...
Current base editors cannot simultaneously edit multiple loci with base-pair level precision, hindering complex genotype generation. Here the authors describe optimized Cas12a gRNA array designs, enab...
