UKRIFLF & professor of biophysics at the University of Sheffield. High-resolution #AFM and #biophysics studies of #DNA structure and function www.pyne-lab.uk 🔬🧬 she/her
Alice Pyne
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It’s been a busy start to the year with marking, paper and grant reviews and travel to SMB2025 in the US, I think we were delayed for as long as we were actually at the conference…
Its good to be home in the Peak District with my best girl Tasso 🌅 🐕 ❤️
Supercoiling not only brings DNA to life (and makes it dance) but also changes how key proteins such as #CRISPR interact with it. Thanks @qmsmith.bsky.social for the brilliant collaboration, Sylvia for developing an amazing new #imageanalysis pipeline & @eddierollins.bsky.social for beautiful #AFM.
After a remarkable 300 columns, David Goodsell has retired from the Molecule of the Month. We are very grateful for his service.
@jiwasa.bsky.social will continue the series, beginning with January's article on Assembly Line Polyketide Synthases
Details: cdn.rcsb.org/rcsb-pd...
📐On #WorldMetrologyDay, we celebrate 150 years of measurement science! At #SPM40, precision at the nanoscale is our mission, advancing biomedical research through AI-powered microscopy. #MetrologyMatters #MSCA #ResearchEU
New research in Nature shows that the physical twisting of DNA plays an important role in DNA editing mistakes made by CRISPR.
Using nanometer-sized circles of DNA, scientists captured never-before-seen interactions between CRISPR & DNA, providing insights that could eradicate errors altogether.
Alice Pyne
Alice Pyne
RCSB Protein Data Bank
Abstract deadlines coming up soon!
Talk - 14th March
Poster - 28th March
Sign up on our Eventbrite: www.eventbrite.co.uk/e/glasgow-im... and come along and see our wonderful keynote speaker @alicepyne.bsky.social !
For more info, head to the GIN website : glasgowimagingnetwork.wordpress.com
Organized with the @sheffielduni.bsky.social team: Matt Newton, @alicepyne.bsky.social & Ruby Peters.
👏 Speakers include Gijs Wuite, @jwelburn.bsky.social, @slfort.bsky.social, @akellett.bsky.social, @michellepeckham.bsky.social & Aakash Basu.
Program & registration --> bit.ly/3NtxZlM
Some of my PhD work is now out in Nature!
www.nature.com/articles/s41...
Investigating the structural basis of off‑target Cas9 binding to supercoiled DNA with #AFM & cryo-EM. From my time in the lab of @alicepyne.bsky.social, in collaboration with @qmsmith.bsky.social and the team at the MRC LMS.
1/11 In our story out today in @nature.com , we showcase the molecular mechanisms that enable Cas9 to bind and cleave negatively supercoiled ((-)SC) off-targets! 🧬🧵
🔗 www.nature.com/articles/s41...
#CRISPR #Cas9 #DNATopology #AFM #CryoEM #SingleMolecule #Biophysics
🚨 PhD position available for Oct 25
🔬Using advanced microscopy to understand how keratin networks control tissue mechanics
An interdisciplinary #cellbiology #biophysics collaboration with Ruby Peters & @alicepyne.bsky.social
👇 Apply by March 14
www.findaphd.com/phds/project...
1/11 In our story out today in @nature.com , we showcase the molecular mechanisms that enable Cas9 to bind and cleave negatively supercoiled ((-)SC) off-targets! 🧬🧵
🔗 www.nature.com/articles/s41...
#CRISPR #Cas9 #DNATopology #AFM #CryoEM #SingleMolecule #Biophysics
After a remarkable 300 columns, David Goodsell has retired from the Molecule of the Month series. Janet Iwasa will be continuing the series for PDB-101.
CRISPR is a powerful DNA-editing tool that has underpinned huge advancements in human healthcare in the last decade. It is a precision tool, but is not perfect, and misplaced DNA edits can compromise ...
lms.mrc.ac.uk
Bringing together those from academia and industry, GIN provides a platform for delegates to share, promote and discuss microscopy.
the Glasgow Imaging Network (GIN) Symposium is back for 2025!
The symposium will take place on 4th April 2025 at the Mazumdar-Shaw Advanced Research Centre (ARC), University of Glasgow.
Register and submit your abstract via Eventbrite:
www.eventbrite.co.uk/e/glasgow-im...
Glasgow Imaging Network
Bringing together those from academia and industry, GIN provides a platform for delegates to share, promote and discuss microscopy.
