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by @danabra.mov
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by @danabra.mov
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by @jimpick.com
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by @atsui.org
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Supercoiling not only brings DNA to life (and makes it dance) but also changes how key proteins such as #CRISPR interact with it. Thanks @qmsmith.bsky.social for the brilliant collaboration, Sylvia for developing an amazing new #imageanalysis pipeline & @eddierollins.bsky.social for beautiful #AFM.
2mo
Alice Pyne
1/11 In our story out today in @nature.com , we showcase the molecular mechanisms that enable Cas9 to bind and cleave negatively supercoiled ((-)SC) off-targets! 🧬🧵 🔗 www.nature.com/articles/s41... #CRISPR #Cas9 #DNATopology #AFM #CryoEM #SingleMolecule #Biophysics
2mo
Cas9 structures explain topology sensing and off-target activation.
www.nature.com
Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity - Nature
Quentin Smith