This Visual-omics approach is kind of amazing: "We resolved each to sufficient
resolution (2.8 and 3.6 Å) to predict the amino acid composition directly from the 3D electrostatic potential map"
including state-of-the-art #CryoET #CryoEM #teamtomo
Excited to be presenting this work today at the 3DEM Gordon Research Seminar and later this week at the Gordon Research Conference. Looking forward to the discussions and feedback!
www.grc.org/three-dimens...
Phylogenetic analysis revealed that the Pam system is widespread exclusively within CPR bacteria, with no homologs identified in non-CPR lineages, indicating that Pam pili are specific to and unique within this superphylum.
This study further highlights the power of integrating advanced cryo-EM with computational structural biology to assign functions to the vast repertoire of proteins encoded within microbial “dark matter,” where conventional annotation approaches often fall short.
The CPR has a very high host specificity! We have not tested just the filament! These are all great experiments!
What remained cryptic was the identity of the filaments that JR1 uses to lock onto their prey. Using cryo-EM, we solved the structure of the filament at high resolution, but most CPR proteins lack obvious sequence homologs and have very poor functional annotation.
