Secreting bacteria undergo drastic changes.
We found that in Yersinia, T3SS activation triggers rapid, large-scale reorganisation of chromosomal and plasmid DNA. This links secretion to growth inhibition-revealing a new connection between virulence and bacterial cell biology.
doi.org/10.1016/j.ce...
Special thanks 🙏 go to first author @ljmihajlovic.bsky.social , as well as Lara Hofacker, Florian Lindner, Priyanikha Jayakumar, and collaborator @diepold-lab.bsky.social !
Our first accepted manuscript at my new lab (@kitkarlsruhe) is out in Communications Biology! 🎉
We show how Pseudomonas aeruginosa coordinates its virulence systems, T3SS, H1-T6SS, flagellum, at single-cell resolution.
đź”— www.nature.com/articles/s42...
@t3sss.bsky.social @NaturePortfolio.bsky.social
This is the first of three manuscripts we’ve recently had accepted — more to come soon! 🚀
#Microbiology #InfectionBiology #SingleCell
Using live-cell microscopy, we uncovered distinct bacterial subpopulations that primarily use certain combinations of these nanomachines:
• some prioritize motility & T3SS
• others shift to T6SS for antagonism
All tuned by the usual suspect: cyclic di-GMP.
What if even the core of bacterial nanomachines wasn’t static?
We found that the T3SS core protein SctD in Yersinia dynamically exchanges subunits — and this flexibility is essential for proper assembly & function.
đź”— www.nature.com/articles/s41...
@kit.edu @t3sss.bsky.social
We recently described how T3SS effectors reach the injectisome with a shuttle system. But what about their chaperones? With live microscopy, proximity labeling and functional assays, @keithpint.bsky.social showed the Yersinia chaperone SycH already hands over its cargo in the cytosol. bit.ly/47hq6rM
Exciting PhD position available in the Diepold lab @kit.edu ! We're especially interested in international students with expertise in molecular biology and ready to lead a cutting-edge research project in molecular machines and infection biology. Find out more at diepoldlab.com.
T3SS activity comes at a cost—bacteria that use the system pay with reduced growth. We found how Yersinia balance costs and benefits: at higher densities, they actively suppress T3SS activity and adhesion, switching from colonization to replication and dissemination.
doi.org/10.1371/jour...
@kit.edu
🛠️Toolbox 🛠️ out to modulate gene expression & protein secretion in a predatory bacterium.
đź“‘Preprint from my group below.
Many thanks to L. Mihajlovic @ljmihajlovic.bsky.social, L. Hofacker, F. Lindner, P. Jayakumar & A. Diepold @diepold-lab.bsky.social! #MicroSky #SyntheticBiology #PredatoryBacteria
