Laser scanning on your equipment entails tradeoffs between speed, resolution, and field-of-view. Pick 2 of those 3.
Now you can get all 3, with this breakthrough.
www.laserfocusworld.com/optics/artic... Thanks @laserfocusworld.bsky.social
“Because scientific knowledge is tentative and provisional, it is the enemy of dogma.” -Baron Taverne
FN > 45
Whatever objective you use, Ventana imaging systems will get more field-of-view out of it, with full NA, and diffraction limited. The best ever for multiphoton / two-photon.
And fast! 16,000 - 24,000 lines/s across the full field of view.
pacificoptica.com/ventana/
“Because [science] is the most effective way of learning about the physical world, it erodes superstition, ignorance and prejudice, which have been at the root of the denial of human rights throughout history…” -Baron Taverne
All Pacific Optica products are designed in Santa Barbara, California, USA.
“Because science rejects claims to truth based on authority and depends on the criticism of established ideas, it is the enemy of autocracy.” -Baron Taverne
Loving the "CEO eats his own burger/hot dog/..." stuff.
Pacific Optica's CEO uses his own Ventana, Diesel2p, and Cousa products. They're the high throughput, turnkey, workhorse systems that power the lab.
pacificoptica.com/ventana/
pacificoptica.com/diesel2p/
pacificoptica.com/cousa/
Huge field-of-view, fast, high res. two-photon imaging. This technology (paper link below) is available now, in the Ventana products.
Sold as complete turnkey systems, or components. pacificoptica.com/ventana/
Installations are happening now at some of the best labs in the world.
An excited customer, sharing photos of the optics they just received.
And one of the first Avocado objectives in customer hands. It's a Cousa-class objective, with an NA of 0.6 and a WD of 10 mm, optimized for 2p, and excellent performance out to the edges of the field-of-view. More info soon!
Cool paper from Garret Stuber's lab and colleagues, led by Madelyn Hjort.
mPFC-VTA circuit for contingency degredation. doi.org/10.1038/s415...
Fig. 3 & 4 used the Cousa objective: **2p imaging + 2p holographic optogenetic stim., deep in brain using microprisms** !
pacificoptica.com/cousa/
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Two-photon calcium imaging at 24,000 lines/s, with the resonant axis spanning 4x what other systems can do. Inertia-free. Diffraction-limited. No tradeoffs. Che-Hang Yu developed a 4x angle multiplier for laser scanning. His paper is out today: opg.optica.org/optica/fullt... 1/n #fluorescenceFriday
