❓What if you could peek between your tilt series ❓ We are proud to introduce cryoTIGER -- deep-learning based tilt interpolation for enhanced reconstruction in cryo-ET. A great collaboration between Turoňová lab, @becklab.bsky.social, @hummerlab.bsky.social #teamtomo www.nature.com/articles/s42...
www.nature.com
Deep learning-based interpolation using cryoTIGER enhances angular sampling in cryoET, improving 3D reconstructions, particle localization, and structural recovery without increasing electron dose.
Ready to TANGO? 💃 In our new publication, Beata Turoňová & Markus Schreiber (@ms314.bsky.social, @becklab.bsky.social) present TANGO: the first cryoET framework to analyze the relation between particle positions and orientations 🪩 All in a single user-friendly package! #CryoET #OpenScience
Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...
Excited to share our latest work on nuclear pore complex scaffold integrity and its perturbation. Now out in Nature Cell Biology: rdcu.be/eg4i9
Great work by Reiya Taniguchi who just opened his own research group back in Japan! 🥳
Jan Philipp Kreysing
My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...!
Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!
Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo
It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...
Out now in Nature Communications!
We show that Dictyostelium vaults associate with ER and NE membranes in situ and encapsulate ribosomes with defined orientations, shining new light on their cellular function! #TeamTomo #TeamMassSpec #ProtistsOnSky
www.nature.com/articles/s41...
Desislava Glushkova
rdcu.be
Nature Cell Biology - Taniguchi et al. structurally analyse nuclear pore complex architecture in situ during differentiation, which is associated with mechanical constraints on the nuclear...
Nuclear pore complexes (NPCs) bridge across the nuclear envelope and mediate nucleocytoplasmic exchange. They consist of hundreds of nucleoporin build…
