Ready to TANGO? 💃 In our new publication, Beata Turoňová & Markus Schreiber (@ms314.bsky.social, @becklab.bsky.social) present TANGO: the first cryoET framework to analyze the relation between particle positions and orientations 🪩 All in a single user-friendly package! #CryoET #OpenScience
Updated architectural model of the NPC from our lab, starred by bsky-less Agnieszka Obarska-Kosińska. Using cryo ET, XL-MS and modeling, we identify novel nucleoporins, among those the mRNA export complex TREX-2! Check out the preprint on biorxiv #TeamMassSpec #TeamTomo
#teamtomo strikes again! Check out our new preprint on the enigmatic vault and its newly discovered association with membranes in situ.
New paper out!
How can we force HIV genomes out of their capsid shells and what can we learn about HIV biology doing so?
Hint: capsid inhibitors actively re-route nuclear HIV complexes, and expose genomes via intriguing structural effects!
open access:
link.springer.com/article/10.1...
read below
We have published a review on how contextual information can complement and enhance research using #cryoET .
www.sciencedirect.com/science/arti...
PhD: defended ✅🎉 My work used in situ cryo-ET #teamtomo to investigate cellular machines from ribosome translation and HIV capsid at the nuclear pore to membrane association of vault, and the molecular architecture of heterochromatin. Huge thanks to @becklab.bsky.social. What a wonderful ride!
We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better.
Preprint: www.biorxiv.org/content/10.6...
Code: github.com/warpem/miss-...
🧶 Thread:
Exciting new paper from my colleague @deglushk.bsky.social in the @becklab.bsky.social! It introduces a fast, robust tool for measuring membrane thickness in tomograms #TeamTomo.
We are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉
Video
Following cell entry, HIV-1 capsids enter the nucleus by passage through nuclear pores and reach nuclear speckles with subsequent uncoating of the reverse-transcribed genome and its integration into s...
Out now in Nature Communications!
We show that Dictyostelium vaults associate with ER and NE membranes in situ and encapsulate ribosomes with defined orientations, shining new light on their cellular function! #TeamTomo #TeamMassSpec #ProtistsOnSky
www.nature.com/articles/s41...
Cryo-electron tomography (cryo-ET) enables the analysis of biological samples in situ, revealing the complex interplay that shapes (sub)cellular archi…
