Excited to share our new paper!
This work explores how Sonic hedgehog signaling helps coordinate retinal patterning and neurogenesis, including regulation of both cell cycle exit and progenitor competence during mammalian eye development journals.biologists.com/dev/article/...
New paper out 🚨! Excited to share this collab between my lab and @kerschensteiner.bsky.social's group, expertly led by Ning Shen. We used a genome-wide in vivo CRISPR screen to uncover novel neuroprotective strategies in the P23H mouse retina, with validation in a new human retina culture model.
Excited to share our new manuscript from the Yoshimatsu and MacDonald labs. www.biorxiv.org/content/10.6.... We found that my favourite glial cells can regulate local retinoic acid signalling to specialise cone photoreceptors for high acuity visual function.
We’ve reached a milestone in the lab! Congrats to Dr. Jade Hostetler for a fantastic thesis defense and becoming the first PhD graduate of the Clark lab! Jade has been leading all of our work on RNA biology including studies of Peanut (Gm11454) pubmed.ncbi.nlm.nih.gov/41573818/ and more to come.
@alainaurman.bsky.social
Identifying genome-wide locations of active DNA demethylation. Studies were performed in an ongoing, fruitful collaboration with John R Edwards and Alaina Urman, helping us integrate the new methylation profiling with previous studies from the Dyer and Nathans labs.
Congrats to @ismaelhdeznunez.bsky.social for taking on this project that included 9 different genotypes of TET mutants, and a special thanks to all other co-authors and collaborators.
Our work on the TET enzymes in retinal development is out. We identified that rod fate is inhibited when DNA demethylation is prevented by removal of the TET enzymes. Interestingly, photoreceptor numbers are normal. We utilized WGBS and bACE-seq to profile the precise localization of 5mC and 5hmC,…
Our new paper is out! 🎉
Let-7 doesn’t act through a single pathway: it controls neocortical neuron fate and migration via distinct downstream mechanisms, with RBX2 dedicated to migration. A new layer of post-transcriptional control shaping cortical development
www.pnas.org/doi/10.1073/...
Happy to share the definitive version of our last paper about the role of TET enzymes and DNA demethylation in retinal development @clark-lab-retina.bsky.social journals.plos.org/plosbiology/...
Summary: Temporal disruption of sonic hedgehog signaling reveals stage-specific roles in eye cup patterning and in regulating proliferation, progenitor competence and fate specification during retinal...
TET enzymes remove #DNAmethylation markers; @ismaelhdeznunez.bsky.social @clark-lab-retina.bsky.social &co show that these enzymes are required for #photoreceptor cells to initiate the genetic program to become rods instead of cones, & for maturation of the #retina @plosbiology.org 🧪 plos.io/3UaVBfl
Shen et al. combine a genome-wide CRISPR screen in mice with an adult human retina model to identify broadly protective gene therapies for retinitis pigmentosa. They show that boosting UFD1 or UXT preserves photoreceptors and vision by helping the retina withstand stress from misfolded proteins.
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
