Happy to see this out now in Cell Genomics: A genome-scale single-cell CRISPRi map of trans gene regulation across human pluripotent stem cell lines: www.cell.com/cell-genomic...
🧬 How do immune disease-relevant variants affect gene regulatory networks in CD4+ T cells?
🧪🖥️ We coupled two large-scale CRISPRi screens (>4M cells) to map the downstream cascades of thousands of SNPs
More details in the thread below 👇 or in the article 📖 on biorxiv tinyurl.com/CD4screens
Insightful seminar by Roderic Guigó: Figure 1 of the Craig Venter human genome paper (Science, 2001) was made by a Spanish PhD student who had no salary and earned his living serving tables in a Pizzeria.
Early bird registration is closing Feb 28! We will have an AMAZING party!
Proud of @juruehle.bsky.social for getting her PhD paper on biorxiv! In it, we take synthetic enhancer screens to the single cell level, offering new insight into how transcription factors and enhancers interact 👀
Delighted to have our work on 🧬 resilience to 🩸cancer led by @g-agarwal.bsky.social & amazing collaborators, including @kharaslab.bsky.social, published in @science.org: www.science.org/doi/10.1126/... 🧵
Expansion cultures of mouse AML stem cells! Enabling large scale genetic screens in a system that's very close to "the real thing", with deep implications for drug discovery. Great work @alejofraticelli.bsky.social and @indrasingh.bsky.social
Now, synthetic enhancers with single cells - great work by @juruehle.bsky.social : www.biorxiv.org/content/10.6...
INTERESTED IN SINGLE-CELL LINEAGE TRACING?
Check our new review @naturerevgenet.bsky.social from the @alejofraticelli.bsky.social lab 🎉🎉
nature.com/articles/s41...
With Victoria Parreno, we tried to update the modern manual for genetic tracing and clonal analysis.
Read link: rdcu.be/e50gy
Feng et al. present the first genome-scale CRISPR interference perturbation map with
scRNA-seq readout across many genetic backgrounds in human pluripotent cells, pioneering
population-scale CRISPR pe...
In this Review, Rodriguez-Fraticelli and Parreno discuss advances in single-cell lineage-tracing methods and how their application to diverse biological processes, such as development, ageing and canc...
ISCO (Innovations in Single-Cell OMICS) will be back in beautiful Barcelona!
🗓️ 28th/29th of May 2026
📍Barcelona Biomedical Research Park @prbb.org (beachfront!)
Keynotes: @bartdeplancke.bsky.social and @bocklab.bsky.social
Submit your abstract and present your research!
www.isco-conference.eu
🚨 NEW
@FraticelliLab
PREPRINT JUST WENT LIVE
biorxiv.org/content/10.6...
Here, we describe PLSTCs, Soluplus-based expansion cultures of primary mouse *Acute Myeloid Leukemia* Stem Cells. And then we use LARRY barcoding to identify drivers of leukemogenicity and drug resistance!
Exactly 25 years ago, @rodericguigo.bsky.social and his PhD student published the first draft of the human genome in Science. The Catalan researchers were the only two from all Spain that took part. Here they are reflecting on the huge scientific feat: www.crg.eu/en/news/25-y...
Out in Cell @cp-cell.bsky.social: Design principles of cell-state-specific enhancers in hematopoiesis
🧬🩸 screen of fully synthetic enhancers in blood progenitors
🤖 AI that creates new cell state specific enhancers
🔍 negative synergies between TFs lead to specificity!
www.cell.com/cell/fulltex...
🧵
Lars Velten
The main project of my PhD 🧬🔬 is out: we developed single-cell lentiMPRA, a lentivirus-based method to measure enhancer activity and transcriptomes at single-cell resolution. We then applied sc-lentiMPRA to fully synthetic enhancers 🧩...
🔗 doi.org/10.64898/202...
Screen of minimalistic enhancers in blood progenitor cells demonstrates widespread
dual activator-repressor function of transcription factors (TFs) and enables the model-guided
design of cell-state-sp...
