6/ 🙏 Huge thanks to the amazing collaborators and co-authors who made this work possible!
🧑🔬 Basile Leduque, Leandro Quadrana, Keith Slotkin, Alexandros Bousios (@abousios.bsky.social) & Hans-wilhelm Nuetzmann
1/📢News from TE diary - Somatic transposition in Arabidopsis is more explosive than we imagined!
Thrilled to share our latest findings - TEs make new somatic insertions en masse, reshaping the somatic genome🧬with surprising frequency🍃
@abousios Hans-Wilhelm Nuetzmann
www.biorxiv.org/content/10.1...
3/ 🧬 Our work shows somatic TE activity in Arabidopsis is non‑random and family‑specific. Different TE families prefer distinct genomic niches - is it driven by underlying epigenetic code, or are they avoiding conflict in a shared genome ecosystem? 🤔
4/ 🔥 Somatic insertion hotspots of the EVADE transposon are enriched at environmentally responsive genes 🌄 - including Resistance gene and biosynthetic gene clusters.
Is stress guiding TE activity, or are TEs tuning stress responses?
Somatic genome plasticity 🧬
2/ Combining distinct experimental conditions, we traced a roadmap of expected TE movement in somatic cells 🌿, capturing ~200K new TE insertions across multiple families in the process.
5/ 💻 Surprise package! We developed a custom pipeline, deNOVOEnrich, to profile somatic TE insertions from TEd-seq short-read data.
Using split-read mapping, we pinpoint new somatic insertions at base-pair resolution 🧬🔍
Check it out: github.com/hAmbreen02/d...