Generate synthetic cryo-ET data and train your model for particle picking in real data.
Impressive work by Mohamad Harastani:
www.nature.com/articles/s41...
A question for the experts: Can you recognize nucleosomes and linker DNA in this data?
Find an updated version of our manuscript in
www.biorxiv.org/content/10.1...
I am not afraid to say it: this is the most fundamental advance in our understanding of chromatin structure since the zig-zag fiber model. Congratulations!
We are in EMBO J. See DNA and non-octameric nucleosome-like particles in situ
link.springer.com/article/10.1...
Using single-H1 imaging and computational modeling, we show that most H1 behaves like a liquid inside chromatin domains rather than binding stably to nucleosomes as in the textbook model:
www.science.org/doi/10.1126/...
Congrats
@masaashimazoe.bsky.social & all contributors! 🎉 2/
Kazuhiro Maeshima
Video
Particle picking is a key bottleneck in bridging structural and cell biology with cryo-electron tomography. Here, authors develop Template Learning, using domain-randomized simulations to train deep l...
Chromatin organization, through the assembly of DNA with histones and the folding of nucleosome chains, regulates DNA accessibility for transcription, DNA replication and repair. Although models deriv...
Our linker histone H1 paper is featured on the cover of the April 10 issue of #ScienceAdvances.
www.science.org/doi/10.1126/...
A molecular dynamics simulation of nucleosomes and linker histone H1, with single-H1 trajectories (background). 🎉 Full cover caption: www.science.org/toc/sciadv/1...
Kazuhiro Maeshima
Our H1 paper is out #ScienceAdvances:
www.science.org/doi/10.1126/...
@masaashimazoe.bsky.social et al. reveal that linker histone H1 acts as a liquid-like glue to organize chromatin in live cells. 🎉 Fantastic collab with @rcollepardo.bsky.social @janhuemar.bsky.social and others—huge thanks! 🙌 1/
