Countless thanks to all our collaborators from the labs of Bradley Bernstein, Albin Sandelin, and @jengreitz.bsky.social.
As an optimised transcription initiation assay 🧪, nucCAGE is more sensitive to nuclear and unstable capped RNAs – crucially allowing us to enrich for sought-after enhancer RNAs. As a proof-of-principle we dissected cell type-specific 🧫 enhancer activities at the human MYC locus.
Dear gene regulation aficionados in the wider Copenhagen 🇩🇰area: Please feel cordially invited to our 3rd Copenhagen Gene Regulation Seminar 👨🏫 next week on Thursday, the 5th of March. 📆 Hope to see you in Panum for 2 exciting talks! 👇
Absolutely none of this would have been possible without the Andersson lab, crucially Hjörleifur Einarsson, Natsuda Navamajiti and of course @randersson.bsky.social. Very proud as well for this to be the first preprint 🖨️ of the Tissue Gene Regulation lab.
In parallel, PRIME predicts active CREs directly from transcription initiation patterns which allowed to build a large-scale atlas 🗺️ of CRE activities across human cell types.🫀🧠🫁👁️
We demonstrate how you can use this atlas 🗺️ together with scE2G-predicted enhancer-to-gene links 🔗 to unravel the gene regulatory landscape 🏔️ of your favourite enhancer, promoter, gene or variant of interest.
Absolutely thrilled 💣 to share our preprint 🖨️: "Mapping active cis-regulatory elements from transcription initiation events”.
www.biorxiv.org/content/10.6...
We introduce nucCAGE and PRIME to identify active cis-regulatory elements (CREs) and disease-associated, non-coding variants.
Germ cells have their own versions of core transcription factors and fertility depends on them.
We're hiring a PhD student to figure out how! 📢
Fly genetics + proteomics + genomics. Fully funded.
Aarhus University 🇩🇰
Deadline May 1 👇
Please share with anyone who might be interested!
www.biorxiv.org
DNA replication globally disrupts the epigenome. But does this create a chromatin-access opportunity for TF binding to facilitate cell identity change? Now 𝐫𝐞𝐩𝐥𝐢-𝐀𝐓𝐀𝐂-𝐬𝐞𝐪 shows 𝘥𝘦 𝘯𝘰𝘷𝘰 chromatin opening & TF binding occurs specifically post-replication, in cellular differentiation & reprogramming!
Visualising heteroplasmic mitochondrial DNA variants in situ? Now out as #mtDNA-smFISH in @cp-devcell.bsky.social! We find purifying selection against pathogenic mtDNA variants through genetic bottlenecks in the germline and the developing brain. www.cell.com/developmenta...
