Daratumumab in lupus 🦋 – results of our single-arm trial:
10 patients 8x daratumumab (1/week) at week 12:
☑️ reduced dsDNA antibodies
☑️ Median SLEDAI 12 > 4
☑️ SRI-4 at 12 weeks 100%
☑️ immunology improved*
out now at @nature.com Communications: www.nature.com/articles/s41...
#medsky #nephsky 🧪 1/
Adapting the 10x workflow to detect EBV reads, Younis et al show that EBV-transcribing B cells are increased in SLE patients, especially among CD27+CD21low B cells. They have autoreactive BCRs and are more poised for antigen-presentation.
2025 Sci Transl Med 🧪 ⑂
www.science.org/doi/10.1126/...
What mechanisms drive checkpoint inhibitor-associated interstitial nephritis (ICI-AIN)?
In our preprint, we identify CD8 T cells as a central player, inducing a proinflammatory myeloid cell state by IFNg and structuring lymphoid aggregates by CXCL13.
www.biorxiv.org/content/10.1...
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#Nephsky 🧪
Damgaard et al – 2016 – The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity in @cellpress.bsky.social
doi.org/10.1016/j.ce...
Ubiquitinylation is more complex than just a signal for proteasomal degradation: Linear ubiquitinylation of Methionine-1 is important for NFkB signalling.
LOF of the Deubiquitinylase OTULIN leads to an autoinflammatory disease with a TNF positive feedback loop.
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Tregs in the skin reduce the activation of sensory neurons by producing enkephalin. This inhibits secondary inflammatory changes mediated by the neurons. (in 🐭)
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Mendoza et al 2025 in Science Immunology
www.science.org/doi/full/10....
“Good things take time”
Allogeneic Treg do not prevent their own rejection in immunocompetent hosts - HLA matching or HLA editing required for optional function!
A beautiful collaboration with Fadi Issa & Joanna Hester’s team @ox.ac.uk. @dlwagnerlabs.bsky.social
Hou, Sun et al from @kuchroolab.bsky.social 2024 in @natureportfolio.nature.com
"Neuropeptide signalling orchestrates T cell differentiation"
www.nature.com/articles/s41...
CRISPR screening factors influencing Th1 vs Th2 differentiation, RAMP3 which forms part of a CGRP receptor is a positive regulator of Th1 differention. CGRP acts by increasing intracellular cAMP and ultimately ATF3 activity it induces STAT1 and thereby Th1 differentiation. in 🐭
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You like engineering T cells, but you are missing the right flavor?
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪