If you like transposons...
If you you love genome editing...
Or if you just like random bird animations,
we have the paper for you!
We (@kedmonds.bsky.social et al) are happy to share our work turning a songbird retrotransposon into a genome editing tool. 🐣 (1/n)
STITCHR, a tool that can make virtually any kind of edit in mammalian genomes including the scarless multi-kilobase scale insertions. Plasmids here www.addgene.org/browse/artic...
Video
Max Wilkinson
Addgene
@cp-molcell.bsky.social
This is the result of a great and ongoing collaboration with the Nishimasu lab. Thanks a bunch to all authors
🚨 Today we report cryo-EM structures of the Bxb1 integrase complexed with its attB/attP DNA substrates before, during and after recombination.
Using these structures, we engineer Bxb1 mutants with improved recombination efficiencies and altered sequence recognition properties.
Check it out! ⬇️
Soma et al. present cryo-EM structures of the large serine recombinase Bxb1 bound
to attP and attB substrates and provide mechanistic insights into attP/attB recognition,
synaptic complex formation, a...
So excited to share our new paper out today in @nature.com. We repurposed R2 retrotransposons to create STITCHR, a tool that can make virtually any kind of edit in mammalian genomes including the scarless multi-kilobase scale insertions 🧵
www.nature.com/articles/s41...
