🚨 Today we report cryo-EM structures of the Bxb1 integrase complexed with its attB/attP DNA substrates before, during and after recombination.
Using these structures, we engineer Bxb1 mutants with improved recombination efficiencies and altered sequence recognition properties.
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Soma et al. present cryo-EM structures of the large serine recombinase Bxb1 bound
to attP and attB substrates and provide mechanistic insights into attP/attB recognition,
synaptic complex formation, a...
