Postdoc @ HHMI Janelia w/ Pachitariu + Stringer Lab. Former PhD at NIH/UMD. 🧠⚡
Paul Kristian LaFosse
Loading...
With raster photostim, we can perform photostim experiments at a large scale, for example by targeting thousands of neurons in a single pattern and updating those patterns on a frame-by-frame basis. 4/n
To enable large-scale expts in vivo, we relied on SOTA viral/transgenic strategies from our collaborators to express GCaMP and super-sensitive ChRmine opsin across the cortex. This allowed us to photostim ~1,000+ neurons across a 3.5 mm FOV using a two-photon mesoscope! 6/n
Huge thanks to all involved, including @ Janelia (jET/Vivarium/MolGen/Viral Tools), MBF, and our collaborators for the amazing viruses/mice! @sverregr.bsky.social, @klensj.bsky.social, @mariannefyhn.bsky.social, Tony Drinnenberg, Charu Ramakrishnan, @deisseroth.bsky.social, and those at Allen /end
1/ 🚨🚨Preprint alert!
"Perfect" isn't always smart 🧠. Anyone can tell apart two stimuli (including our mice). But how do we extract rules that apply to new stimuli? 🧵
biorxiv.org/content/10.6...
@marius10p.bsky.social @computingnature.bsky.social
#neuroAI #neuroscience #AI
While there are tradeoffs to using raster photostim (e.g. no simultaneous activation like holo photostim), we hope this tool helps to simplify and increase access for others wanting to do all-optical experiments, especially at a large-scale/across different brain regions! 7/n
🚨 Ever wanted a simple way to control the activity of thousands of neurons? We introduce an all-optical raster photostimulation method to do just that! w/ @computingnature.bsky.social @marius10p.bsky.social #FluorescenceFriday #neuroskyence
www.biorxiv.org/content/10.6... 1/n
Similarly, raster photostimulation could be used at a much smaller scale to target individual dendrites or groups of dendrites of a single neuron. 5/n
Our solution: instead of a complex/separate optical path, we route our ⚡ ️laser through the same resonant-scanning path as the imaging laser and simply turn it on/off over neurons/areas of interest. This allows for pixel-resolution accuracy, and ability to ⚡ ️anywhere in the FOV you can image. 3/n
We were driven by a desire to easily ⚡️ large groups of neurons to study computations requiring coordinated activity across the 🧠
Tools exist to photostim select patterns of neurons, e.g. holographic stim, but these can be a bit restrictive; both in # of neurons, but also in setup/maintenance. 2/n
My travel to Cosyne was barred by the Trump admin, so I'm here on my personal dime. I care about the COSYNE community, I committed to co-chairing. And I always learn here.
But the worst part about this travel ban is my lab colleagues— students and fellows—couldn't come. /1
