The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-proteinaceous substrates in vitro https://www.biorxiv.org/content/10.1101/2024.11.29.626046v1
Excited to share our latest work, now out in @lsajournal.org. we show that the E3 ligase HOIL-1 can ubiquitinate many different saccharides and describe a chemoenzymatic platform to generate ubiquitinated sugars as tools for studying non-proteinaceous ubiquitination.
📣📣 Lorne Ubiquitin – The Australian Ubiquitin Summit 📣📣 20-23 Nov 2025 in Lorne, Australia Join us this Nov for the first ubiquitin meeting in Australia. Submit your abstract by 18 July 2025 for oral and poster presentations: www.lorneubiquitin.org.
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We hope this will be useful for the field. A plasmid for expressing the constitutively active HOIL-1 variant is available via @addgene.bsky.social: www.addgene.org/229539/
We are still accepting abstracts for selected short talks and posters. The abstract deadline has been extended to this Friday, 1st Aug. More information and registration here: www.lorneubiquitin.org .
For those interested in ubiquitinating their own sugars: our plasmid for bacterial expression of the constitutive HOIL-1 variant is now available via Addgene: www.addgene.org/229539/
HOIL-1 is a RING-between-RING (RBR)-family E3 ubiquitin ligase and component of the linear ubiquitin
Plasmid pOPINB-M1-di-Ub(G76V)-HOIL-1 from Dr. Bernhard Lechtenberg's lab contains the inserts Ubiquitin, Ubiquitin, and HOIL-1 and is published in bioRxiv November 30, 2024 This plasmid is available ...
Plasmid pOPINB-M1-di-Ub(G76V)-HOIL-1 from Dr. Bernhard Lechtenberg's lab contains the inserts Ubiquitin, Ubiquitin, and HOIL-1 and is published in bioRxiv November 30, 2024 This plasmid is available ...
📣📣 Lorne Ubiquitin – The Australian Ubiquitin Summit 📣📣 20-23 Nov 2025 in Lorne, Australia Join us this Nov for the first ubiquitin meeting in Australia. Submit your abstract by 18 July 2025 for oral and poster presentations: www.lorneubiquitin.org.
Please share and repost.
The RBR E3 ligase HOIL-1 ubiquitinates Ser/Thr-residues and various disaccharides in vitro and can be used to generate ubiquitinated sugars as tools to study non-proteinaceous ubiquitination
@lechtenberglab.bsky.social @wehi-research.bsky.social
www.life-science-alliance.org/content/8/6/...
Excited to share our latest preprint on @biorxivpreprint.bsky.social: "The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-protein substrates in vitro".
www.biorxiv.org/content/10.1...
Lechtenberg Lab
HOIL-1 is a RING-between-RING (RBR)-family E3 ubiquitin ligase and component of the linear ubiquitin chain assembly complex (LUBAC). While most E3 ubiquitin ligases conjugate ubiquitin to protein lysi...
