For those interested in ubiquitinating their own sugars: our plasmid for bacterial expression of the constitutive HOIL-1 variant is now available via Addgene: www.addgene.org/229539/
Plasmid pOPINB-M1-di-Ub(G76V)-HOIL-1 from Dr. Bernhard Lechtenberg's lab contains the inserts Ubiquitin, Ubiquitin, and HOIL-1 and is published in bioRxiv November 30, 2024 This plasmid is available ...
Excited to share our latest preprint on @biorxivpreprint.bsky.social: "The RBR E3 ubiquitin ligase HOIL-1 can ubiquitinate diverse non-protein substrates in vitro".
www.biorxiv.org/content/10.1...
HOIL-1 is a RING-between-RING (RBR)-family E3 ubiquitin ligase and component of the linear ubiquitin chain assembly complex (LUBAC). While most E3 ubiquitin ligases conjugate ubiquitin to protein lysi...
