The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes:
🖌️ Dozens more images with duplications or painting
🖨️ Hundreds of blots that all share the same background (behold slideshow below)
Video
While this is newly out of date by one article, here is a record of the past articles
blogs.nature.com/methagora/20...
The #ThermoFisher #antibody scandal is an ugly reminder, I think, of how few people (including those at TF, it seems) are trained to properly validate antibodies before use. Some advice on antibody validation in the posting 👇
totalinternalreflectionblog.com/2022/09/18/h...
Update on GeneFrame alternative for bacterial imaging. After discussion with us and others, SunJin lab have how launched a substitute for timelapse imaging in geneframes www.sunjinlab.com/product/ispa...
Dimensions are very similar, price is reasonable ($105 for 250) #microsky
George Campbell
Oh and of course the editorial that was not supposed to be shared:
diabetesjournals.org/care/article...
I feel like I am echoing the editorial, but the Points of View and Points of Significance series were very influential to my scientific journey. I am thrilled to see Points of View come back, being helmed by @helenajambor.bsky.social who has already done great work amongst the microscopy community
Reese Richardson
Antibodies are essential and ubiquitous tools in biology, but their unguarded usage can easily lead to error. Here’s how to validate their specificity, and ensure that they’re hitting the rig…
and a collection of the sibling series (and a few more), Points of Significance - www.nature.com/collections/...
translated: we used faked results to try and convince you to buy our over-priced antibodies, and then offer a set of lies to justify it
Total Internal Reflection🇬🇧🇺🇸🇺🇦
Thermo Fisher has responded to research integrity activists who spotted apparent manipulation in its antibody catalog:
"In ... preparing antibody images for publication on our website, some images may have been adjusted to clarify for presentation purposes."
More:
cen.acs.org/research-int...
The opinions expressed in this editorial are the personal views of the authors (S.E. Kahn, C.A.M. Anderson, J.B. Buse, and E. Selvin) and do not represent
The Thermo Fisher situation keeps getting worse. We've now collected 450+ problematic images presented as verification data in TF's antibody catalog. This includes:
🖌️ Dozens more images with duplications or painting
🖨️ Hundreds of blots that all share the same background (behold slideshow below)
A collection of articles from the publisher of Nature that discusses statistical issues biologists should be aware of and provides practical advice to improve the statistical rigor and reproducibility...
Thermo Fisher has responded to research integrity activists who spotted apparent manipulation in its antibody catalog:
"In ... preparing antibody images for publication on our website, some images may have been adjusted to clarify for presentation purposes."
More:
cen.acs.org/research-int...
Reese Richardson
Mark Peifer (He, him)
Laurel Oldach
Fellow bacterial cell biologists, message me if you use @thermofishersci.bsky.social Gene Frames for microscopy!
Thermo are planning to discontinue these in September but have indicated they will look into this further.
If I make list of labs using them Thermo might not kill the product
Please RT🔬🦠
We are very excited to bring back our popular Points of View column, written by @helenajambor.bsky.social! Check out her first column in our July issue about the usage of color scales in scientific figures.
www.nature.com/articles/s41...
42.1K Views. University of Groningen. This protocol provides a step-by-step procedure to monitor single cell behavior of different bacteria in time using automated fluorescence time-lapse microscopy....
