NAD to the bone: How bacteria put phages under aRES-t … and how phages fight back
Preview of work IDing aRES, bacterial proteins that deplete cellular NAD+, generating products that cannot be utilized by phage NAD+ regeneration pathways
www.cell.com/cell-host-mi...
How do pathogens disarm plant immunity?
We discovered a widespread strategy used by plant bacterial pathogens: they directly cleave a conserved immune signaling molecule produced by plant immune receptors.
www.biorxiv.org/content/10.6...
Phage receptor-binding proteins are known for their LEGO-like modularity, but we still do not fully understand their evolutionary potential and how it shapes phage host range. Our new preprint examines this systematically in Klebsiella phages. 🔗 www.biorxiv.org/content/10.6...
Thread 🧵
How do human cells defend against viruses?
@sgfern.bsky.social discovers that human immune proteins named ISGs target ancient features of replication shared between animal and bacterial viruses – opening analysis of human immunity to the power of bacterial genetics
www.biorxiv.org/content/10.6...
@ohadroth.bsky.social and @soreklab.bsky.social discover bacterial enzymes that cleave the immune signal 2'cADPR explaining the mystery of XopQ virulence proteins in plant pathogens. Beautiful results mirroring phage inhibition of 2'- and 3'cADPR in bacteria! 🌱✂️
www.biorxiv.org/content/10.6...
Very happy to share this preview article written by my postdoc Danny on exciting recent work from @ostermanilya.bsky.social and @soreklab.bsky.social. New bacterial immunity, phage countermeasures, and a double pun combo in the title.
Folddisco is now published @natbiotech.nature.com. It’s a fast motif search for similar 3D DISCOntinuous residues like catalytic sites or zinc fingers across the entire protein universe.
📄 www.nature.com/articles/s41...
💾 folddisco.foldseek.com
🌐 https://search.foldseek.com/folddisco
Amazing work bridging plant and bacterial immunity together🌱🧫
Congratulations Ohad!
In this issue of Cell Host & Microbe, Osterman et al. discover aRES, a new family
of bacterial immune proteins that deplete cellular NAD+, generating cleavage products
that cannot be utilized by canon...
Our paper is out in Nature Microbiology: functional analysis of 80 phage sponges, revealing new sponges that inhibit bac immunity by binding the immune signals cCMP, cUMP and N7-cADPR
Thanks to all coauthors and our collaborators at the Kranzusch Lab. Congrats Romi!
www.nature.com/articles/s41...
Preprint: Plant pathogens cleave 2’cADPR to suppress TIR immune signaling
Inspired by knowledge on how phages inhibit bacterial immunity, we now show that disease-causing bacteria inhibit plant immunity in a similar way. A 🧵
www.biorxiv.org/content/10.6...
Cell Host & Microbe
Ohad Roth
Kranzusch Lab
Folddisco enables protein structural motif search in million scale databases.
NAD to the bone: How bacteria put phages under aRES-t … and how phages fight back
Preview of work IDing aRES, bacterial proteins that deplete cellular NAD+, generating products that cannot be utilized by phage NAD+ regeneration pathways
www.cell.com/cell-host-mi...
Rafal Mostowy
A functional screen reveals phage sponge proteins that bind Pycsar, Thoeris and CBASS signalling molecules to inhibit bacterial immunity.
In this issue of Cell Host & Microbe, Osterman et al. discover aRES, a new family
of bacterial immune proteins that deplete cellular NAD+, generating cleavage products
that cannot be utilized by canon...
Small molecules produced by TIR (Toll-interleukin-1 receptor) domains are essential for plant immune signaling. A central TIR-derived signal is 2'cADPR, a cyclic ADP-ribose (ADPR) molecule that is gen...
Folddisco finds similar (dis)continuous 3D motifs in large protein structure databases. Its efficient index enables fast uncharacterized active site annotation, protein conformational state analysis and PPI interface comparison. 1/9🧶🧬
📄 www.biorxiv.org/content/10.1...
🌐 search.foldseek.com/folddisco
Video
How do pathogens disarm plant immunity?
We discovered a widespread strategy used by plant bacterial pathogens: they directly cleave a conserved immune signaling molecule produced by plant immune receptors.
www.biorxiv.org/content/10.6...
Excited to see our work out in @natmicrobiol.nature.com! We uncovered broad functional diversity within phage sponge families🧽.
Huge thanks to all coauthors and brilliant collaborators @kranzuschlab.bsky.social @reneechang.bsky.social !
www.nature.com/articles/s41...
Martin Steinegger 🇺🇦
www.biorxiv.org
Ohad Roth
A functional screen reveals phage sponge proteins that bind Pycsar, Thoeris and CBASS signalling molecules to inhibit bacterial immunity.
📢 Preprint out!
Together with @reneechang.bsky.social @kranzuschlab.bsky.social and the amazing @soreklab.bsky.social, we explored viral sponges to map their diversity and function.
Discovered huge diversity, including sponges that inhibit Pycsar & Type IV Thoeris!
www.biorxiv.org/content/10.1...
Multiple bacterial immune systems, including CBASS, Thoeris, and Pycsar, employ signaling molecules that activate the immune response following phage infection. Phages counteract bacterial immune sign...
