Spectral multiplexing is typically limited to 4-5 channels. Our new preprint introduces a framework that utilizes DNA barcoding and signal tuning to enable robust spectral unmixing and ground-truth benchmarking to achieve 15-plex subcellular profiling without cycling
www.biorxiv.org/content/10.6...
Sinem Saka
Ever felt constrained by the "4-color limit" in fluorescence imaging?
We use DNA-barcoded labeling to build high-plex panels, enabling imaging of 15 targets across the full fluorescence spectrum.
doi.org/10.64898/202...
Grateful to @sinemsaka.bsky.social and all authors for making this possible.
