New paper out 💥 3D cell-cell contact reconstitution with synthetic vesicles and (primary) immune cells to study how different proteins and carbohydrates influence the immune synapse dynamics.
advanced.onlinelibrary.wiley.com/doi/10.1002/...
Exciting to see this out in its final form! Wonderful work led by @probablycarmen.bsky.social. Want to know why protrusions are better at initiating immune signaling in #Tcells? Look no further. Link to Carmen’s 🧵 on the preprint bsky.app/profile/prob...
Excited to share our latest work in @ScienceAdvances! 🔬 We’ve developed "dynaMIET"—a new spectroscopy technique that finally allows us to simultaneously measure 3D membrane dynamics in live cells with nanometer precision. www.science.org/doi/10.1126/...
Super excited to share a project I’ve worked on since my Master’s thesis, what a journey!🚵
Finally out as a Nature Methods Stage 1 Registered Report preprint doi.org/10.6084/m9.f...
We benchmark deep learning-based single-image super-resolution methods for microscopy imaging.
Interested? Check out 🧵
Now out in its final form in @jacs.acspublications.org: Reaction pathways & photoreaction yields of bifunctional lipid probes. The numbers that explain why we spend so much time optimizing protocols for cellular assays. 😉 pubs.acs.org/doi/10.1021/...
Excited to share our #preprint showing that #macrophages in the regenerating #zebrafish #heart are shaped by local cardiac-immune microniches, with a fibroblast-to-macrophage signal promoting regeneration over fibrotic repair www.biorxiv.org/content/10.6...
@idrm.ox.ac.uk @oxforddpag.bsky.social
@nadlerlab.bsky.social strikes again! Lipid-CLEM by @mathilda95.bsky.social and co..
dynaMIET enables simultaneous measurement of lateral diffusion and vertical membrane fluctuations in live cells.
Virtual super-resolution deep learning methods provide a powerful solution to overcome the physical and temporal constraints of microscopy imaging. Yet, assessing and choosing an ideal methodological strategy complicates their use in life sciences and creates a lack of trust in these methods. Here we propose an objective comparison of nine popular single-image super-resolution (SISR) models in a collection of publicly available microscopy datasets, including cell components like microtubules, endoplasmic reticulum, and actin, using confocal microscopy, SEM, SIM, SMLM and STED microscopy modalities for fixed and live-cell microscopy data. The proposed models will be assessed quantitatively with a collection of metrics in microscopy and computer vision, and qualitatively by experts in the field. The proposed models will be made accessible through open, user-friendly, containerised notebooks. This systematic assessment of SISR approaches will provide a more comprehensive understanding of these methods' performance and contribute to standardising SISR methods in microscopy.
Our WE-Heraeus seminar "Fluorescence markers for advanced microscopy" at the Ecole de physique des Houches is now over ! Thanks to my amazing co-organizers Ulrike Endesfelder, Viktorija Glembockyte and Dominique Bourgeois.
@glembockyte.bsky.social
@uendesfelder.bsky.social
Bifunctional diazirine lipids are versatile tools for mapping protein–lipid interactions and cellular localization by photo-cross-linking. Yet, the cross-linking efficiency of these probes has not bee...
It's always important to carefully characterize the reactivity of your favorite tools to optimize applications. We teamed up with Carla Kirschbaum and Carol V. Robinson to map photolysis reaction pathways of bifunctional lipids www.biorxiv.org/content/10.6...
Emmanuel Margeat
Revised role for actin-rich protrusions in T-cell activation:
@franbottanelli.bsky.social @probablycarmen.bsky.social et al reframe them as sites of enhanced receptor activation by exclusion & clustering dynamics, rather than pre-enrichment of signaling components
link.springer.com/article/10.1...
www.biorxiv.org
link.springer.com
Actin-rich protrusions densely cover the surface of T cells and are well characterised for their role in migration. Recent studies have uncovered their contribution to antigen surveillance and immune ...
It’s incredibly hard to study lipids in biological membranes on the nanoscale. You need near-perfect information on both membrane ultrastructure and lipid density. Lipid-CLEM, now out in @natcellbio.nature.com brought to you by @mathilda95.bsky.social changes that:
www.nature.com/articles/s41...
The EMBO Journal
André Nadler
Looks like a great @embo.org workshop coming in September: “Lipid Code to Life”, with a who’s who of lipid #biochemistry… (e.g. @dimovalab.bsky.social @holthuislab.bsky.social @sciezgin.bsky.social etc ) More info and registration: meetings.embo.org/event/26-lip... #lipids #biophysics
Modern lipid biology has evolved far beyond the traditional view of lipids as simple building blocks or energy substrates. Lipids are now recognised as dynamic regulators of biological structures and…
