🎉 Big news! Last Saturday, Thierry was awarded the Egon Naef Foundation Prize for in vitro research 🏆 A wonderful recognition of his innovative work throughout his career developing alternatives to animal testing 👏
www.unige.ch/sciences/chi...
@sciencesunige.bsky.social
Very excited to share our recently published work on NTM and their response to acid stress!
As my first paper, I’m especially grateful for the support and guidance from @luizcarvalholab.bsky.social , Rachel and @maxgg.bsky.social . More to come!
We then pushed it further
🦠 Tracking M. marinum-induced damage in Dicty
🧫 Monitoring Gal3 recruitment after LLOMe in mammalian cells
To monitor membrane damage & repair in real time, we developed a robust high-throughput approach combining:
📸 live high-content imaging
📊 automated quantification
🧬 stable, homogeneous reporter cell lines
As a proof of concept ✨
We used Dictyostelium cells expressing two ESCRT reporters: Alix & Vps32.
→ Our approach tracks their kinetics after LLOMe-induced damage and confirms earlier recruitment of Alix after damage
🧵 Tiny organisms, big advantages!
How does the mycobacterial vacuole go from damage that can still be repaired to full rupture? 👀
Our new preprint shows that EsxA and PDIM act sequentially: EsxA initiates repairable lesions, and PDIM helps push them past host repair capacity toward cytosolic escape.
doi.org/10.64898/202...
For more details, check out the chapter 👇
onlinelibrary.wiley.com/doi/10.1002/...
And to see the method in action 👇
www.science.org/doi/10.1126/...
💥Our latest @embojournal.org on membrane damage and repair. Di @dichen02.bsky.social @crick.ac.uk found that calcium is a conserved signal for ATG8/LC3 lipidation on membranes using novel probes to record calcium leakage. Congratulations Di et al!
link.springer.com/article/10.1...