Tuberculosis🔬 - Postdoc at the Crick 🇬🇧 - SoldatiLab Unige alumni 🇨🇭
Angélique Perret
Loading...
For more details, check out the chapter 👇
onlinelibrary.wiley.com/doi/10.1002/...
And to see the method in action 👇
www.science.org/doi/10.1126/...
As a proof of concept ✨
We used Dictyostelium cells expressing two ESCRT reporters: Alix & Vps32.
→ Our approach tracks their kinetics after LLOMe-induced damage and confirms earlier recruitment of Alix after damage
🧵 Tiny organisms, big advantages!
We then pushed it further
🦠 Tracking M. marinum-induced damage in Dicty
🧫 Monitoring Gal3 recruitment after LLOMe in mammalian cells
To monitor membrane damage & repair in real time, we developed a robust high-throughput approach combining:
📸 live high-content imaging
📊 automated quantification
🧬 stable, homogeneous reporter cell lines
How does the mycobacterial vacuole go from damage that can still be repaired to full rupture? 👀
Our new preprint shows that EsxA and PDIM act sequentially: EsxA initiates repairable lesions, and PDIM helps push them past host repair capacity toward cytosolic escape.
doi.org/10.64898/202...
Angélique Perret
🎉 Big news! Last Saturday, Thierry was awarded the Egon Naef Foundation Prize for in vitro research 🏆 A wonderful recognition of his innovative work throughout his career developing alternatives to animal testing 👏
www.unige.ch/sciences/chi...
@sciencesunige.bsky.social
Very excited to share our recently published work on NTM and their response to acid stress!
As my first paper, I’m especially grateful for the support and guidance from @luizcarvalholab.bsky.social , Rachel and @maxgg.bsky.social . More to come!
Angélique Perret
Angélique Perret
Angélique Perret
Angélique Perret
Soldati Lab
Soldati Lab
🚨 New preprint!
Together with @carobarisch.bsky.social lab, we investigated how intracellular compartmentalization shapes lipid acquisition in mycobacteria
Interestingly, deleting one FACL enzyme (FACL6) revealed striking compartment-dependent phenotypes 🔬
Check it out: doi.org/10.64898/202...
💥Our latest @embojournal.org on membrane damage and repair. Di @dichen02.bsky.social @crick.ac.uk found that calcium is a conserved signal for ATG8/LC3 lipidation on membranes using novel probes to record calcium leakage. Congratulations Di et al!
link.springer.com/article/10.1...
Parise Lockwood
Video
Mélanie Foulon
Max Gutierrez
Endomembrane damage of intracellular vesicles triggers signals that activate membrane repair in mammalian cells to restore homeostasis. However, the signals that drive diverse membrane repair recruitm...
